2. Academic Validation
  3. Parental exposure to Cypermethrin causes intergenerational toxicity in zebrafish offspring

Parental exposure to Cypermethrin causes intergenerational toxicity in zebrafish offspring

  • Sci Total Environ. 2024 May 22:935:173456. doi: 10.1016/j.scitotenv.2024.173456.
Shuang Han 1,  Xi Liu 2,  Yixiang Liu 3,  Jian Lu 4
Affiliations

Affiliations

  • 1 Morphology and Spatial Multi-omics Technology Platform, Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences, Yueyang Road 320, 200031 Shanghai, China; Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
  • 2 Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
  • 3 State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, China. Electronic address: lyxcm@126.com.
  • 4 State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, China; Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. Electronic address: jianlu@ynau.edu.cn.
PMID: 38788937 DOI: 10.1016/j.scitotenv.2024.173456
Abstract

Cypermethrin (CYP), a synthetic pyrethroid pesticide, has been detected in agriculture and aquaculture. However, there is limited knowledge about the transgenerational impacts. This study aimed to investigate the developmental toxicity of CYP on F1 larvae offspring of adult zebrafish exposed to various CYP concentrations (5, 10, and 20 μg/L) for 28 days. The results indicated that CYP accumulated in parental zebrafish, and CYP was below the limit of quantification in offspring. Paternal exposure impacted the hatching rate and heart rate of the F1 generation. Furthermore, CYP significantly impacted the development of swim bladders in progeny and dysregulated the genes relevant to swim bladder development. The neutrophil migrated to the swim bladder. The mRNA levels of the inflammatory factors were also significantly elevated. According to network toxicology, PI3-AKT may be the signaling pathway for CYP-influenced bladder development. Subsequent molecular docking and Western blot analysis showed CYP affected the PI3-AKT signaling pathway. Notably, MK-2206, a specific Akt Inhibitor, rescued the CYP-induced damage of swim bladder development in offspring. The present study highlights the potential risks of CYP on the development of offspring and lasting impact in aquatic environments.

Keywords

Cypermethrin; Development toxicity; Intergenerational transmission; Swim bladder; Zebrafish.

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