2. Academic Validation
  3. Design of "Off-On-Off" fluorescence sensors for Heparin detection by precise modulation of molecular structure

Design of "Off-On-Off" fluorescence sensors for Heparin detection by precise modulation of molecular structure

  • Spectrochim Acta A Mol Biomol Spectrosc. 2024 Jun 8:320:124630. doi: 10.1016/j.saa.2024.124630.
Yanan Li 1,  Jian Cao 2,  Chuanxiao Wang 1,  Yiyi Qiao 1,  Jiayu Gao 1,  Xiao Zhang 1,  Le Wang 1
Affiliations

Affiliations

  • 1 School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, 333 Longteng Road, Shanghai 201620, PR China.
  • 2 School of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, 333 Longteng Road, Shanghai 201620, PR China. Electronic address: caoj@sues.edu.cn.
PMID: 38865888 DOI: 10.1016/j.saa.2024.124630
Abstract

In this strategy, the fluorescence sensor Nap-Co-T1 employing the fluorescence resonance energy transfer (FRET) mechanism was designed and synthesized to have an efficient response to Heparin, and the FRET mechanism was explored for different excitation-emission wavelengths with different distances between the energy acceptor and the energy donor (comparing with fluorescence sensor Nap-TPA-T2). Upon the addition of Heparin, the fluorescence emission of Nap-Co-T1 was turned on at 565 nm, and the fluorescence color changed of the solution from colorless to bright yellow. The limit of detection (LOD) was as low as 0.04 μg/mL. With the addition of antagonistic protamine (PRTM) to the sensor complex with Heparin, the fluorescence emission was turned off to a certain extent, and the reversibility of the "off-on-off" system was maintained for five cycles or more. In addition, Nap-Co-T1 provides rapid and sensitive detection of Heparin in human serum albumin solution and artificial urine and is highly sensitive to environmental viscosity.

Keywords

Artificial urine; FRET mechanism; Fluorescence; Heparin; Sensor; Viscosity.

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