1. Academic Validation
  2. RUNX1 regulates MCM2/CDC20 to promote COAD progression modified by deubiquitination of USP31

RUNX1 regulates MCM2/CDC20 to promote COAD progression modified by deubiquitination of USP31

  • Sci Rep. 2024 Jun 17;14(1):13906. doi: 10.1038/s41598-024-64726-w.
Wei Tian # 1 2 Jingyuan Zhao # 3 Xinyu Zhang # 1 2 Pengfei Li 1 2 Xuening Li 2 Yuan Hong 4 Shuai Li 5
Affiliations

Affiliations

  • 1 The First Affiliated Hospital of Dalian Medical University, Dalian, China.
  • 2 Dalian Medical University, Dalian, China.
  • 3 Stem Cell Clinical Research Center, The First Affiliated Hospital of Dalian Medical University, Dalian, China.
  • 4 Clinical Laboratory Center, Dalian Municipal Central Hospital, Dalian, China. yuanhonglab@163.com.
  • 5 Department of Pharmacy, The First Affiliated Hospital of Dalian Medical University, Dalian, China. shuaili2015@outlook.com.
  • # Contributed equally.
Abstract

Colon adenocarcinoma (COAD) is the second leading cause of Cancer death, and there is still a lack of diagnostic biomarkers and therapeutic targets. In this study, bioinformatics analysis of the TCGA database was used to obtain RUNX1, a gene with prognostic value in COAD. RUNX1 plays an important role in many malignancies, and its molecular regulatory mechanisms in COAD remain to be fully understood. To explore the physiological role of RUNX1, we performed functional analyses, such as CCK-8, colony formation and migration assays. In addition, we investigated the underlying mechanisms using transcriptome Sequencing and chromatin immunoprecipitation assays. RUNX1 is highly expressed in COAD patients and significantly correlates with survival. Silencing of RUNX1 significantly slowed down the proliferation and migratory capacity of COAD cells. Furthermore, we demonstrate that CDC20 and MCM2 may be target genes of RUNX1, and that RUNX1 may be physically linked to the deubiquitinating Enzyme USP31, which mediates the upregulation of RUNX1 protein to promote transcriptional function. Our results may provide new insights into the mechanism of action of RUNX1 in COAD and reveal potential therapeutic targets for this disease.

Keywords

Colon adenocarcinoma; Prognosis; RUNX1; Transcription factors; USP31.

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