2. Academic Validation
  3. ISIR and its human homolog gene AK131315 strengthen LPS-induced inflammation and acute lung injury by promoting TAK1-dependent NF-κB and MAPK signaling

ISIR and its human homolog gene AK131315 strengthen LPS-induced inflammation and acute lung injury by promoting TAK1-dependent NF-κB and MAPK signaling

  • Int Immunopharmacol. 2024 Jun 18:137:112510. doi: 10.1016/j.intimp.2024.112510.
Tao Sun 1,  Famin Wang 2,  Jiashan Li 3,  Wei Wei 2,  Yan Wang 3,  Zhaowei Tong 4,  Weihua Zou 5
Affiliations

Affiliations

  • 1 Clinical Laboratory, Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University, Huzhou 313000, Zhejiang, PR China; Huzhou Key Laboratory of Precision Medicine Research and Translation for Infectious Diseases, Huzhou Central Hospital, Huzhou 313000, Zhejiang, PR China.
  • 2 The Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou 313000, Zhejiang, PR China.
  • 3 Clinical Laboratory, Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University, Huzhou 313000, Zhejiang, PR China.
  • 4 Huzhou Key Laboratory of Precision Medicine Research and Translation for Infectious Diseases, Huzhou Central Hospital, Huzhou 313000, Zhejiang, PR China; Department of Infectious Diseases, Huzhou Central Hospital, Huzhou 313000, Zhejiang, PR China. Electronic address: hztongzhaowei@163.com.
  • 5 Clinical Laboratory, Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University, Huzhou 313000, Zhejiang, PR China; Huzhou Key Laboratory of Precision Medicine Research and Translation for Infectious Diseases, Huzhou Central Hospital, Huzhou 313000, Zhejiang, PR China; Clinical Laboratory, Huzhou Central Hospital, Affiliated Central Hospital of Huzhou University, Huzhou 313000, Zhejiang, PR China. Electronic address: zxyyjykzwh@126.com.
PMID: 38897130 DOI: 10.1016/j.intimp.2024.112510
Abstract

Acute lung injury (ALI), a critical complication observed in various clinical disorders, is characterized by widespread inflammation, neutrophil infiltration, and proinflammatory cytokine production. This study showed that the recently identified non-coding RNA ISIR and its human homolog gene AK131315 played a role in regulating lipopolysaccharide (LPS)-induced inflammatory responses. ISIR and AK131315 increased the production of inflammatory cytokines in LPS-stimulated macrophages, and exogenous ISIR aggravated LPS-induced lung inflammation in an animal model of ALI. Mechanistically, ISIR promoted LPS-triggered NF-κB and MAPK signaling and the transcription of proinflammatory cytokines by enhancing TAK1 activation. Furthermore, a significant correlation was observed between AK131315 expression and pulmonary infectious caused by Gram-negative bacteria, suggesting that AK131315 plays an important role in Bacterial infections. Altogether, these findings indicate that ISIR regulates LPS-induced inflammation and AK131315 is involved in the pathogenesis of Bacterial infections.

Keywords

AK131315; Acute lung injury; ISIR; Inflammatory response; TAK1.

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