2. Academic Validation
  3. Biofabrication and Monitoring of a 3D Printed Skin Model for Melanoma

Biofabrication and Monitoring of a 3D Printed Skin Model for Melanoma

  • Adv Healthc Mater. 2024 Jul 11:e2401136. doi: 10.1002/adhm.202401136.
Paula Vázquez-Aristizabal 1 2,  Malou Henriksen-Lacey 2 3,  Clara García-Astrain 2 3,  Dorleta Jimenez de Aberasturi 2 3 4,  Judith Langer 2 3,  Claudia Epelde 5,  Lucio Litti 6,  Luis M Liz-Marzán 2 3 4 7,  Ander Izeta 1 8
Affiliations

Affiliations

  • 1 Stem Cells and Aging Group, Biogipuzkoa Health Research Institute, Paseo Dr. Begiristain s/n, Donostia-San Sebastián, 20014, Spain.
  • 2 CIC biomaGUNE, Basque Research and Technology Alliance (BRTA), Paseo de Miramón 194, Donostia-San Sebastián, 20014, Spain.
  • 3 Centro de Investigación Biomédica en Red, Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Donostia-San Sebastián, 20014, Spain.
  • 4 Ikerbasque Basque Foundation for Science, Bilbao, 48009, Spain.
  • 5 Obstetrics and Gynaecology Service, Donostia University Hospital, Paseo Dr. Begiristain s/n, Donostia-San Sebastián, 20014, Spain.
  • 6 Department of Chemical Sciences, University of Padova, Via Marzolo, 1, Padova, 35131, Italy.
  • 7 Cinbio, Universidade de Vigo, Campus Universitario, Vigo, 36310, Spain.
  • 8 School of Engineering, Tecnun-University of Navarra, Donostia-San Sebastián, 20009, Spain.
PMID: 38992996 DOI: 10.1002/adhm.202401136
Abstract

There is an unmet need for in vitro Cancer Models that emulate the complexity of human tissues. 3D-printed solid tumor micromodels based on decellularized extracellular matrices (dECMs) recreate the biomolecule-rich matrix of native tissue. Herein a 3D in vitro metastatic melanoma model that is amenable for drug screening purposes and recapitulates features of both the tumor and the skin microenvironment is described. Epidermal, basement membrane, and dermal biocompatible inks are prepared by means of combined chemical, mechanical, and enzymatic processes. Bioink printability is confirmed by rheological assessment and bioprinting, and bioinks are subsequently combined with melanoma cells and dermal fibroblasts to build complex 3D melanoma models. Cells are tracked by confocal microscopy and surface-enhanced Raman spectroscopy (SERS) mapping. Printed dECMs and cell tracking allow modeling of the initial steps of metastatic disease, and may be used to better understand melanoma cell behavior and response to drugs.

Keywords

3D bioprinting; SERS; dECM; melanoma; metastasis.

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