2. Academic Validation
  3. Long-term high fructose intake reprograms the circadian transcriptome and disrupts homeostasis in mouse extra-orbital lacrimal glands

Long-term high fructose intake reprograms the circadian transcriptome and disrupts homeostasis in mouse extra-orbital lacrimal glands

  • Exp Eye Res. 2024 Jul 16:246:110008. doi: 10.1016/j.exer.2024.110008.
Di Qi 1,  Duliurui Huang 2,  Mengru Ba 2,  Shuting Xuan 3,  Hongli Si 2,  Dingli Lu 1,  Xiaoting Pei 1,  Wenxiao Zhang 2,  Shenzhen Huang 1,  Zhijie Li 4
Affiliations

Affiliations

  • 1 Henan Eye Institute, Henan Eye Hospital and Henan Key Laboratory of Ophthalmology and Visual Science, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, 450000, China.
  • 2 Department of Ophthalmology, Zhengzhou University People's Hospital, Henan Provincial People's Hospital, Zhengzhou, Henan, 450000, China.
  • 3 Department of Ophthalmology, Henan University People's Hospital, Henan Provincial People's Hospital, Zhengzhou, Henan, 450000, China.
  • 4 Henan Eye Institute, Henan Eye Hospital and Henan Key Laboratory of Ophthalmology and Visual Science, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, 450000, China. Electronic address: zhijielee@vip.163.com.
PMID: 39025460 DOI: 10.1016/j.exer.2024.110008
Abstract

This study aims to explore the effects of long-term high fructose intake (LHFI) on the structure, functionality, and physiological homeostasis of mouse extra-orbital lacrimal glands (ELGs), a critical component of ocular health. Our findings reveal significant reprogramming of the circadian transcriptome in ELGs following LHFI, alongside the activation of specific inflammatory pathways, as well as metabolic and neural pathways. Notably, LHFI resulted in increased inflammatory infiltration, enhanced lipid deposition, and reduced nerve fiber density in ELGs compared to controls. Functional assessments indicated a marked reduction in lacrimal secretion following cholinergic stimulation in LHFI-treated mice, suggesting impaired gland function. Overall, our results suggest that LHFI disrupts lacrimal gland homeostasis, potentially leading to dry eye disease by altering its structure and secretory function. These insights underscore the profound impact of dietary choices on ocular health and highlight the need for strategies to mitigate these risks.

Keywords

Extra-orbital lacrimal glands; High fructose intake; Inflammation; Metabolism; Neuron; RNA-Seq.

Figures
Products