2. Academic Validation
  3. Cytoplasmic binding partners of the Integrator endonuclease INTS11 and its paralog CPSF73 are required for their nuclear function

Cytoplasmic binding partners of the Integrator endonuclease INTS11 and its paralog CPSF73 are required for their nuclear function

  • Mol Cell. 2024 Aug 8;84(15):2900-2917.e10. doi: 10.1016/j.molcel.2024.06.017.
Min-Han Lin 1,  Madeline K Jensen 2,  Nathan D Elrod 3,  Hsu-Feng Chu 1,  MaryClaire Haseley 4,  Alissa C Beam 4,  Kai-Lieh Huang 2,  Wesley Chiang 4,  William K Russell 3,  Kelsey Williams 5,  Christoph Pröschel 5,  Eric J Wagner 6,  Liang Tong 7
Affiliations

Affiliations

  • 1 Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
  • 2 Department of Biochemistry and Biophysics, Center for RNA Biology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, TX 77550, USA.
  • 3 Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, TX 77550, USA.
  • 4 Department of Biochemistry and Biophysics, Center for RNA Biology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.
  • 5 Department of Biochemistry and Biophysics, Center for RNA Biology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA; Department of Biomedical Genetics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.
  • 6 Department of Biochemistry and Biophysics, Center for RNA Biology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA; Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, TX 77550, USA. Electronic address: eric_wagner@urmc.rochester.edu.
  • 7 Department of Biological Sciences, Columbia University, New York, NY 10027, USA. Electronic address: ltong@columbia.edu.
PMID: 39032490 DOI: 10.1016/j.molcel.2024.06.017
Abstract

INTS11 and CPSF73 are metal-dependent endonucleases for Integrator and pre-mRNA 3'-end processing, respectively. Here, we show that the INTS11 binding partner BRAT1/CG7044, a factor important for neuronal fitness, stabilizes INTS11 in the cytoplasm and is required for Integrator function in the nucleus. Loss of BRAT1 in neural organoids leads to transcriptomic disruption and precocious expression of neurogenesis-driving transcription factors. The structures of the human INTS9-INTS11-BRAT1 and Drosophila dIntS11-CG7044 complexes reveal that the conserved C terminus of BRAT1/CG7044 is captured in the active site of INTS11, with a cysteine residue directly coordinating the metal ions. Inspired by these observations, we find that UBE3D is a binding partner for CPSF73, and UBE3D likely also uses a conserved cysteine residue to directly coordinate the active site metal ions. Our studies have revealed binding partners for INTS11 and CPSF73 that behave like cytoplasmic chaperones with a conserved impact on the nuclear functions of these Enzymes.

Keywords

3′-end processing; Integrator; chaperone; endonuclease; transcription termination.

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