2. Academic Validation
  3. Dendrobine Ameliorates Glucocorticoid-Induced Osteoporosis by Promoting Osteogenesis through JNK/p38 MAPK Pathway Activation and GR Nuclear Translocation Inhibition

Dendrobine Ameliorates Glucocorticoid-Induced Osteoporosis by Promoting Osteogenesis through JNK/p38 MAPK Pathway Activation and GR Nuclear Translocation Inhibition

  • J Agric Food Chem. 2024 Jul 31;72(30):16739-16748. doi: 10.1021/acs.jafc.4c02798.
Xufeng Li 1,  Chenyi Gao 2,  Ke Zhou 1,  Kaifeng Gan 1,  Tianjie Ye 3,  Jun Zhao 4,  Jin Li 1,  Chi Ma 4
Affiliations

Affiliations

  • 1 Department of Orthopedic Surgery, the Affiliated Lihuili Hospital of Ningbo University, Ningbo 315040, China.
  • 2 Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
  • 3 Department of Rehabilitation Medicine, the Affiliated Lihuili Hospital of Ningbo University, Ningbo 315040, China.
  • 4 Department of Orthopedics, the First Affiliated Hospital of Jishou University, Jishou 416000, Hunan, China.
PMID: 39033544 DOI: 10.1021/acs.jafc.4c02798
Abstract

Glucocorticoid-induced osteoporosis (GIOP) is the common reason for secondary osteoporosis. Dendrobine (DEN) is the major biologically active component of Dendrobium officinale with anti-inflammatory and antiaging properties. Whether DEN could alleviate osteogenic inhibition in GIOP rats is still unknown. The influence on osteogenic function caused by DEN on dexamethasone-treated bone marrow mesenchymal stem cells and rats was observed. The in vitro results showed that DEN reversed the inhibition of osteogenic differentiation by dexamethasone. Moreover, DEN supplementation attenuated dexamethasone-induced bone loss in vivo. DEN activated JNK and p38 MAPK pathways and restrained GR nuclear translocation, which could be prevented by the JNK (SP600125) or p38 (SB203580) pathway inhibitor. This study verified that DEN alleviated dexamethasone-induced nuclear translocation of GR, and inhibition of osteogenesis via JNK and p38 pathways, laying the foundation for DEN as a therapeutic agent for GIOP.

Keywords

JNK and p38 pathway; glucocorticoid; osteogenic differentiation; osteoporosis.

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