2. Academic Validation
  3. 3D Spheroid and Organoid Models to Study Neuroinfection of RNA Viruses

3D Spheroid and Organoid Models to Study Neuroinfection of RNA Viruses

  • Methods Mol Biol. 2024:2824:409-424. doi: 10.1007/978-1-0716-3926-9_26.
Vinicius Pinho Dos Reis 1,  Karsten Cirksena 2,  Agnieszka Rybak-Wolf 3,  Bettina Seeger 4,  Eva Herker 5,  Gisa Gerold 6 7 8
Affiliations

Affiliations

  • 1 Institute of Virology, Philipps-University Marburg, Marburg, Germany.
  • 2 Department of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany.
  • 3 Organoid Platform, Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC) in the Helmholtz Association, Berlin, Germany. agnieszka.rybak@mdc-berlin.de.
  • 4 Institute for Food Quality and Safety, University of Veterinary Medicine Hannover, Hannover, Germany. bettina.seeger@tiho-hannover.de.
  • 5 Institute of Virology, Philipps-University Marburg, Marburg, Germany. eva.herker@uni-marburg.de.
  • 6 Department of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany. Gisa.Gerold@tiho-hannover.de.
  • 7 Department of Clinical Microbiology, Umeå University, Umeå, Sweden. Gisa.Gerold@tiho-hannover.de.
  • 8 Wallenberg Centre for Molecular Medicine (WCMM), Umeå University, Umeå, Sweden. Gisa.Gerold@tiho-hannover.de.
PMID: 39039427 DOI: 10.1007/978-1-0716-3926-9_26
Abstract

Three-dimensional culture models of the brain enable the study of neuroinfection in the context of a complex interconnected cell matrix. Depending on the differentiation status of the neural cells, two models exist: 3D spheroids also called neurospheres and cerebral organoids. Here, we describe the preparation of 3D spheroids and cerebral organoids and give an outlook on their usage to study Rift Valley fever virus and other neurotropic viruses.

Keywords

3D spheroid; Cerebral organoid; Induced pluripotent stem cells; Neural progenitor cells; Neuroinfection; Neurosphere; Neurotropic virus.

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