2. Academic Validation
  3. Pec 1 of Pseudomonas aeruginosa Inhibits Bacterial Clearance of Host by Blocking Autophagy in Macrophages

Pec 1 of Pseudomonas aeruginosa Inhibits Bacterial Clearance of Host by Blocking Autophagy in Macrophages

  • ACS Infect Dis. 2024 Aug 9;10(8):2741-2754. doi: 10.1021/acsinfecdis.4c00096.
Xiao Jin 1,  Changwen Zhang 2,  Shaoqing Lin 3,  Tianming Gao 1,  Huimin Qian 4,  Lili Qu 5,  Jing Yao 1,  Xingran Du 6,  Ganzhu Feng 1
Affiliations

Affiliations

  • 1 Department of Pulmonary and Critical Care Medicine, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, China.
  • 2 Department of Pulmonary and Critical Care Medicine, The Fourth Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, China.
  • 3 Department of Pulmonary and Critical Care Medicine, The 900th Hospital of the Joint Logistic Support Force, Fujian Medical University, Fuzhou, Fujian 350000, China.
  • 4 Department of Acute Infectious Disease Prevention and Control, NHC Key Laboratories of Enteric Pathogenic Microbiology, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu 210000, China.
  • 5 Laboratory Medicine Center, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, China.
  • 6 Department of Pulmonary and Critical Care Medicine, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing, Jiangsu 210000, China.
PMID: 39047963 DOI: 10.1021/acsinfecdis.4c00096
Abstract

Pseudomonas aeruginosa (P. aeruginosa), a common opportunistic pathogen, is highly prone to chronic Infection and is almost impossible to eradicate, especially attributed to virulence factors and adaptive mutations. In the present study, pseudomonas effector candidate 1 (Pec 1), a novel virulence factor of P. aeruginosa, was investigated, which inhibited Bacterial clearance by the host and aggravated lung injury. Further, it demonstrated that Pec 1 inhibited miR-155 via suppressing Integrin β3 expression, thereby activating PI3K-AKT-mTOR and inhibiting Autophagy in macrophages. Additionally, the identification of Pec 1 in sputum was related to the Bacterial load and assisted in rapid diagnosis of P. aeruginosa Infection. This finding underlined the importance of Pec 1 in the pathogenesis of P. aeruginosa Infection and indicated that Pec 1 could be a vital independent virulence factor during chronic Infection with P. aeruginosa, providing new insights in rapid diagnosis, therapeutic targets, and vaccine antigens of P. aeruginosa Infection.

Keywords

Pseudomonas aeruginosa; autophagy; diagnosis; macrophages; pseudomonas effector candidate 1.

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