1. Academic Validation
  2. Histidine Kinase QseC in Glaesserella parasuis Enhances the Secretion of Proinflammatory Cytokines by Macrophages via the p38 and NF-κB Signaling Pathways

Histidine Kinase QseC in Glaesserella parasuis Enhances the Secretion of Proinflammatory Cytokines by Macrophages via the p38 and NF-κB Signaling Pathways

  • DNA Cell Biol. 2024 Sep;43(9):474-481. doi: 10.1089/dna.2024.0078.
Xuefeng Yan 1 Congwei Gu 2 Zehui Yu 2 Mingde Zhao 2 Lvqin He 2
Affiliations

Affiliations

  • 1 School of Physical Education, Southwest Medical University, Luzhou, China.
  • 2 Technology Department, Experimental Animal Center, Southwest Medical University, Luzhou, China.
Abstract

The qseC gene is a two-component system that encodes a histidine protein kinase and is highly conserved among different Glaesserella parasuis strains. In this study, we used qRT-PCR and enzyme-linked immunosorbent assay to confirm that Toll-like Receptor 4 (TLR4) plays a role in the expression of proinflammatory cytokines interleukin (IL)-1β and IL-6 by stimulating RAW 264.7 macrophages with QseC. Furthermore, we revealed that blocking the p38 and NF-κB pathways that regulate signaling can significantly reduce the production of proinflammatory cytokines induced by QseC. In summary, our data suggest that QseC is a novel proinflammatory mediator that induces TLR4-dependent proinflammatory activity in RAW 264.7 macrophages through the p38 and NF-κB pathways.

Keywords

Glaesserella parasuis; QseC; innate immune system; proinflammatory cytokines; signaling pathway.

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