2. Academic Validation
  3. Lipocalin-2 inhibition alleviates neural injury by microglia ferroptosis suppression after experimental intracerebral hemorrhage in mice via enhancing ferritin light chain expression

Lipocalin-2 inhibition alleviates neural injury by microglia ferroptosis suppression after experimental intracerebral hemorrhage in mice via enhancing ferritin light chain expression

  • Biochim Biophys Acta Mol Basis Dis. 2024 Oct;1870(7):167435. doi: 10.1016/j.bbadis.2024.167435.
Xiaowei Fei 1,  Yanan Dou 1,  Yuefan Yang 1,  Buyi Zheng 2,  Peng Luo 1,  Shuhui Dai 1,  Jingwei Zhang 3,  Kang Peng 4,  Xiaofan Jiang 1,  Yang Yu 1,  Jialiang Wei 5
Affiliations

Affiliations

  • 1 Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
  • 2 Department of Neurosurgery, Wenzhou People's Hospital, Wenzhou, Zhejiang, China.
  • 3 Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China.
  • 4 Department of Radiology, Xiangya Hospital, Central South University, Changsha, China.
  • 5 Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China; Department of Health Service, Fourth Military Medical University, Xi'an, China. Electronic address: Kimi_wei@126.com.
PMID: 39067535 DOI: 10.1016/j.bbadis.2024.167435
Abstract

Introduction: Microglia play pivotal roles in post-intracerebral hemorrhage (ICH) neural injury. Iron metabolism, which is dysregulated after ICH, participates in microglial dysfunction. Previous studies have shown that iron metabolism-related lipocalin-2 (LCN2) is involved in regulating microglial function following ICH. In this study, we investigated the role of LCN2 in microglial function following ICH.

Methods: The BV2 (microglia) cell line, transfected with LCN2 for overexpression/interference, received a blood infusion from C57BL/6 mice in vitro. For the in vivo study of LCN2 function, an LCN2 knockout was conducted in mice. Liproxstatin-1 and RSL3 were used to manipulate Ferroptosis and to study the effects of LCN2 on microglia after ICH. A BV2 (microglia) cell line, transfected with ferritin light chain (FTL) for overexpression/interference, was co-cultured with primary cultured neurons for a study on the mechanism of LCN2. Behavioral tests were conducted pre-ICH and on days 3, 7, and 28 post-ICH, and the brains and cultured cells were collected for protein, histological, and morphological studies.

Results: Brain LCN2 expression was upregulated in microglia, astrocytes, and neurons and played hazardous roles after ICH. In microglia, LCN2 promoted Ferroptosis, which facilitated neural injury after ICH. LCN2-mediated FTL deficiency was shown to be responsible for microglial ferroptosis-induced neural injury.

Conclusion: Our study suggests that LCN2-enhanced microglial Ferroptosis plays a detrimental role by inducing FTL deficiency after ICH. The current study reveals a novel molecular mechanism involved in the pathophysiological progression of ICH.

Keywords

Ferritin light chain; Ferroptosis; Intracerebral hemorrhage; Lipocalin-2; Microglia.

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