2. Academic Validation
  3. Methyl-CpG-binding 2 K271 lactylation-mediated M2 macrophage polarization inhibits atherosclerosis

Methyl-CpG-binding 2 K271 lactylation-mediated M2 macrophage polarization inhibits atherosclerosis

  • Theranostics. 2024 Jul 8;14(11):4256-4277. doi: 10.7150/thno.94738.
Liangqi Chen 1 2,  Meiju Zhang 1 2,  Xueyan Yang 1 2,  Yanan Wang 1 2,  Tuo Huang 1 2,  Xin Li 1 2,  Yunting Ban 1 2,  Qifeng Li 1 2,  Qingyuan Yang 1 2,  Yongxiang Zhang 1 2,  Yang Zheng 1 2,  Di Wang 1 2,  Xiaoqi Wang 1 2,  Xiujie Shi 3,  Maomao Zhang 1 2,  Yong Sun 1 2,  Jian Wu 1 2 4
Affiliations

Affiliations

  • 1 Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
  • 2 The Key Laboratory of Myocardial Ischemia, Chinese Ministry of Education, Harbin, China.
  • 3 The Clinical Skills Center, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
  • 4 Cardiac Rehabilitation Center, Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
PMID: 39113793 DOI: 10.7150/thno.94738
Abstract

Rationale: Posttranslational modifications of proteins have not been addressed in studies aimed at elucidating the cardioprotective effect of exercise in atherosclerotic Cardiovascular Disease (ASCVD). In this study, we reveal a novel mechanism by which exercise ameliorates atherosclerosis via lactylation. Methods: Using ApoE-/- mice in an exercise model, proteomics analysis was used to identify exercise-induced specific lactylation of MeCP2 at lysine 271 (K271). Mutation of the MeCP2 K271 lactylation site in aortic plaque macrophages was achieved by recombinant adenoviral transfection. Explore the molecular mechanisms by which motility drives MeCP2 K271 lactylation to improve plaque stability using ATAC-Seq, CUT &Tag and Molecular Biology. Validation of the potential target RUNX1 for exercise therapy using Ro5-3335 pharmacological inhibition. Results: we showed that in ApoE-/- mice, methyl-CpG-binding protein 2 (MeCP2) K271 lactylation was observed in aortic root plaque macrophages, promoting pro-repair M2 macrophage polarization, reducing the plaque area, shrinking necrotic cores, reducing plaque lipid deposition, and increasing collagen content. Adenoviral transfection, by introducing a mutant at lysine 271, overexpressed MeCP2 K271 lactylation, which enhanced exercise-induced M2 macrophage polarization and increased plaque stability. Mechanistically, the exercise-induced atheroprotective effect requires an interaction between MeCP2 K271 lactylation and H3K36me3, leading to increased chromatin accessibility and transcriptional repression of RUNX1. In addition, the pharmacological inhibition of the transcription factor RUNX1 exerts atheroprotective effects by promoting the polarization of plaque macrophages towards the pro-repair M2 phenotype. Conclusions: These findings reveal a novel mechanism by which exercise ameliorates atherosclerosis via MeCP2 K271 lactylation-H3K36me3/RUNX1. Interventions that enhance MeCP2 K271 lactylation have been shown to increase pro-repair M2 macrophage infiltration, thereby promoting plaque stabilization and reducing the risk of atherosclerotic Cardiovascular Disease. We also established RUNX1 as a potential drug target for exercise therapy, thereby providing guidance for the discovery of new targets.

Keywords

H3K36me3; MeCP2 lactylation; atherosclerosis; chromatin accessibility; exercise; macrophages polarization.

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