2. Metabolic Enzyme/Protease Anti-infection Apoptosis
  3. Hexokinase HSV Apoptosis
  4. 2-Deoxy-D-glucose

2-Deoxy-D-glucose  (Synonyms: 2-DG; 2-Deoxy-D-arabino-hexose; D-Arabino-2-deoxyhexose)

Cat. No.: HY-13966 Purity: 99.93%
COA Handling Instructions

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2-désoxy-D-glucose est un analogue du glucose qui agit comme un inhibiteur compétitif du métabolisme du glucose, inhibant la glycolyse via ses actions sur l'hexokinase.

2-Deoxy-D-Glucose ist ein Glucoseanalogon, das als kompetitiver Inhibitor des glycolysis wirkt und über seine Wirkung auf die hexokinase die Glycolyse hemmt.

2-Deoxy-D-glucose is a glucose analog that acts as a competitive inhibitor of glucose metabolism, inhibiting glycolysis via its actions on hexokinase.

For research use only. We do not sell to patients.

2-Deoxy-D-glucose Chemical Structure

2-Deoxy-D-glucose Chemical Structure

CAS No. : 154-17-6

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5 g USD 192 In-stock
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Based on 165 publication(s) in Google Scholar

Other Forms of 2-Deoxy-D-glucose:

2-Deoxy-D-glucose Related Antibodies

Top Publications Citing Use of Products

163 Publications Citing Use of MCE 2-Deoxy-D-glucose

WB

    2-Deoxy-D-glucose purchased from MedChemExpress. Usage Cited in: Ann N Y Acad Sci. 2023 Mar 18.  [Abstract]

    2-Deoxy-D-glucose (2-DG; 200 μM) reduces the protein levels of CASP11, cleaved-CASP11, NLRP3, GSDMD-FL, and GSDMD-NT, accompanied by effective inhibition of the markers involved in glycolysis, in OCCM-30 cells.

    2-Deoxy-D-glucose purchased from MedChemExpress. Usage Cited in: Oncotarget. 2017 Jul 4;8(27):44669-44681.  [Abstract]

    Effect of 2-DG treatment on BRSK2 and other components of AKT/mTOR signaling pathway. PANC-1 cells are treated with series concentrations of 2-DG for three hours before analysis.

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    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    2-Deoxy-D-glucose is a glucose analog that acts as a competitive inhibitor of glucose metabolism, inhibiting glycolysis via its actions on hexokinase[1].

    IC50 & Target[1]

    HSV-1

     

    In Vitro

    2-Deoxy-D-glucose (2-DG, 4, 8, or 16 mM) significantly reduces the level of ATP in MCF-7 cells in a dose- and time-dependent manner that paralleles the effects of 2-DG on cell growth. The levels of phosphorylated Akt are significantly decreased, whereas the levels of phosphorylated AMPK and Sirt-1 are significantly increased in MCF-7 cells exposed to 2-Deoxy-D-glucose at 4, 8, or 16 mM for 1, 3, or 5 days in a dose- and time-dependent manner[1]. 2-DG treatment increases the levels of pentose phosphate pathway (PPP) metabolites and augments the generation of NADPH by glucose-6-phosphate dehydrogenase. An increase in NADPH and upregulation of glutathione synthetase expression resultes in the increase in the reduced form of glutathione by 2-DG in NB4 cells[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    2-Deoxy-D-glucose Related Antibodies
    In Vivo

    2-Deoxy-D-glucose (0.03%, w/w) causes a 7% decrease in final weight that is statistically significant, and delayes the appearance of palpable mammary carcinomas[1]. 2-Deoxy-D-glucose (3 mmol/kg, i.v.) is decreased in a dose-dependent manner in rat muscle[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    164.16

    Formula

    C6H12O5
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OC1O[C@@H]([C@@H](O)[C@H](O)C1)CO
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 24 mg/mL (146.20 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.0916 mL 30.4581 mL 60.9162 mL
    5 mM 1.2183 mL 6.0916 mL 12.1832 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 130 mg/mL (791.91 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.93%

    COA (248 KB) RP-HPLC (206 KB) Elemental Analysis Report (107 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    The effect of 2-DG on cell growth is determined by evaluating the number of adherent cells. Briefly, MCF-7 cells are plated at 3×104 cells per well in flat-bottomed 96-well plates in 100 μL of culture medium under the culture conditions. After 24 hours, cells are fed with fresh medium including 2-Deoxy-D-glucose at doses of 0, 4, 8, or 16 mM. At days 1, 3, and 5 after 2-Deoxy-D-glucose exposure, cells are fixed with 1% glutaraldehyde, replaced with PBS, and stored at 4°C. At the end of an experiment, all of the plates are stained with 0.02% aqueous crystal violet for 30 minutes and rinsed with deionized water. After redissolving the bound crystal violet in 70% ethanol, the absorbance is determined at 590 nm using a SPECTRA MAX PLUS Microplate Spectrophotometer System.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1]

    At 21 days of age, rats are injected with 50 mg 1-methyl-1-nitrosourea per kilogram of body weight (i.p.). Rats are housed two per cage in solid-bottomed polycarbonate cages equipped with a food cup. Six days following carcinogen injection, all rats are randomized into one of three groups, 30 rats per group, and are fed ad libitum AIN-93G diet containing 0.0%, 0.02%, or 0.03% (w/w) 2-Deoxy-D-glucose (2-DG) for 5 weeks. Animal rooms are maintained at 22±1°C with 50% relative humidity and a 12-hour light/12-hour dark cycle. Rats are weighed thrice per week and are palpated for detection of mammary tumors twice per week starting from 19 days postcarcinogen.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 6.0916 mL 30.4581 mL 60.9162 mL 152.2904 mL
    5 mM 1.2183 mL 6.0916 mL 12.1832 mL 30.4581 mL
    10 mM 0.6092 mL 3.0458 mL 6.0916 mL 15.2290 mL
    15 mM 0.4061 mL 2.0305 mL 4.0611 mL 10.1527 mL
    20 mM 0.3046 mL 1.5229 mL 3.0458 mL 7.6145 mL
    25 mM 0.2437 mL 1.2183 mL 2.4366 mL 6.0916 mL
    30 mM 0.2031 mL 1.0153 mL 2.0305 mL 5.0763 mL
    40 mM 0.1523 mL 0.7615 mL 1.5229 mL 3.8073 mL
    50 mM 0.1218 mL 0.6092 mL 1.2183 mL 3.0458 mL
    60 mM 0.1015 mL 0.5076 mL 1.0153 mL 2.5382 mL
    80 mM 0.0761 mL 0.3807 mL 0.7615 mL 1.9036 mL
    100 mM 0.0609 mL 0.3046 mL 0.6092 mL 1.5229 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    2-Deoxy-D-glucose Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    2-Deoxy-D-glucose154-17-62-DG 2-Deoxy-D-arabino-hexose D-Arabino-2-deoxyhexoseHexokinaseHSVApoptosisHerpes simplex virusInhibitorinhibitorinhibit

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