2. Academic Validation
  3. Blue light photobiomodulation induced osteosarcoma cell death by facilitating ferroptosis and eliciting an incomplete tumor cell stress response

Blue light photobiomodulation induced osteosarcoma cell death by facilitating ferroptosis and eliciting an incomplete tumor cell stress response

  • J Photochem Photobiol B. 2024 Sep:258:113003. doi: 10.1016/j.jphotobiol.2024.113003.
Jiali Yang 1,  Qiqi Fu 1,  Hui Jiang 2,  Hongyu Zhong 3,  Hao Kuan Qin 2,  Xiaojing Miao 1,  Yinghua Li 4,  Muqing Liu 5,  Jinghui Yao 6
Affiliations

Affiliations

  • 1 School of information science and technology, Fudan University, 2005th Songhu Road, Shanghai 200438, China.
  • 2 Academy for Engineering and Technology, Fudan University, 220th Handan Road, Shanghai 200433, China.
  • 3 Department of Pediatric Orthopedic, Center for Orthopedic Surgery, The Third School of Clinical Medicine, The Third Affiliated Hospital of Southern Medical University, No.183, Zhongshan Avenue West, Guangzhou 510515, China.
  • 4 Shanghai Fifth People's Hospital, Fudan University, 801th Heqing Road, Shanghai 200240, China. Electronic address: zhulihan@fudan.edu.cn.
  • 5 School of information science and technology, Fudan University, 2005th Songhu Road, Shanghai 200438, China; Zhongshan Fudan Joint Innovation Center, 6th Xiangxing Road, Zhongshan 28403, China. Electronic address: mqliu@fudan.edu.cn.
  • 6 Department of Pediatric Orthopedic, Center for Orthopedic Surgery, The Third School of Clinical Medicine, The Third Affiliated Hospital of Southern Medical University, No.183, Zhongshan Avenue West, Guangzhou 510515, China. Electronic address: yaojinghui@smu.edu.cn.
PMID: 39121719 DOI: 10.1016/j.jphotobiol.2024.113003
Abstract

To investigate the potential of blue LIGHT photobiomodulation (PBM) in inducing Ferroptosis, a novel form of regulated cell death, in OS cells, considering its known effectiveness in various Cancer Models. In this investigation, we exposed human OS cell lines, HOS and MG63, to different wavelengths (420, 460 and 480 nm) of blue LIGHT at varying irradiances, and examined cellular responses such as viability, Apoptosis, levels of Reactive Oxygen Species (ROS), and mitochondrial membrane potential (MMP). Transcriptome Sequencing was employed to unravel the molecular mechanisms underlying blue light-induced effects, with validation via quantitative Real-Time PCR (qRT-PCR). Our findings revealed a wavelength- and time-dependent decrease in cell viability, accompanied by increased Apoptosis and oxidative stress. Transcriptomic analysis identified differential expression of genes associated with Ferroptosis, oxidative stress, and iron metabolism, further validated by qRT-PCR. These results implicated Ferroptosis as a significant mechanism in the blue light-induced death of OS cells, potentially mediated by ROS generation and disruption of iron homeostasis. Also, An incomplete stress response was observed in MG63 cells induced by blue LIGHT exposure. Hence, blue LIGHT PBM holds promise as a therapeutic approach in OS clinical investigations; however, additional exploration of its underlying mechanisms remains imperative.

Keywords

Apoptosis; Blue light PBM; Ferroptosis; Osteosarcoma; ROS.

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