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  3. Exosomes derived from endothelial progenitor cells ameliorate LPS-induced brain microvascular endothelial cells injury by delivering miR-126a-5p

Exosomes derived from endothelial progenitor cells ameliorate LPS-induced brain microvascular endothelial cells injury by delivering miR-126a-5p

  • Sci Rep. 2024 Aug 9;14(1):18469. doi: 10.1038/s41598-024-69163-3.
Hongquan Zhang 1 2,  Caiyun Lu 3,  Lili Wu 4,  Jiang Li 2,  Min Huang 5,  Xingyu Tao 6,  Yuanbo Wu 7,  Baohui Jia 8 9
Affiliations

Affiliations

  • 1 Department of Respiratory and Critical Care Medicine, The Fourth Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330003, Jiangxi, China.
  • 2 Henan Province Natural Medicine Extraction and Medical Technology Application Engineering Research Center, Zhengzhou, 451460, Henan, China.
  • 3 Zhengzhou Central Hospital, Zhengzhou, 450007, Henan, China.
  • 4 Department of Respiratory and Critical Care Medicine, The University of Hong Kong-Shenzhen Hospital, Shenzhen, 518000, China.
  • 5 Department of Respiratory and Critical Care Medicine, The First Hospital of Nanchang, Nanchang, 330008, Jiangxi, China.
  • 6 Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, 330003, Jiangxi, China.
  • 7 Department of Anesthesiology, Hubei Cancer Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430079, Hubei, China.
  • 8 Department of Respiratory and Critical Care Medicine, The Fourth Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330003, Jiangxi, China. zh885x@126.com.
  • 9 Henan Province Natural Medicine Extraction and Medical Technology Application Engineering Research Center, Zhengzhou, 451460, Henan, China. zh885x@126.com.
PMID: 39122748 DOI: 10.1038/s41598-024-69163-3
Abstract

Endothelial progenitor cells (EPCs) play a crucial role in maintaining vascular health and aiding in the repair of damaged blood vessels. However, the specific impact of EPCs-derived exosomes on vascular endothelial cell injury caused by lipopolysaccharide (LPS) remains inadequately understood. This study aims to explore the potential benefits of EPC-exosomes in mitigating LPS-induced vascular injury and to elucidate the underlying mechanism. Initially, EPCs were isolated from mouse peripheral blood, and their identity was confirmed through flow cytometry and immunocytochemistry. Subsequently, the exosomes derived from EPCs were identified using transmission electron microscopy (TEM) and western blot analysis. A sepsis model was induced by subjecting brain microvascular endothelial cells (BMECs) to LPS-induced injury. Both EPC and their exosomes demonstrated a significant increase in BMECs proliferation, reduced Apoptosis, decreased levels of pro-inflammatory factors (TNF-α, IL-6, and Caspase-3), and enhanced sprouting and angiogenesis of BMECs. Notable, the Exosomes demonstrated a more pronounced impact on these parameters. Furthermore, both EPCs and Exosomes exhibited significantly increased levels of miR-126a-5p, with the Exosomes showing a more substantial enhancement. These findings suggest that supplementing exosomal miR-126a-5p from EPCs can provide protective effects on BMECs, offering a potential therapeutic option for treating sepsis-induced microvascular endothelial cell injury.

Keywords

Endothelial progenitor cells; Exosomes; LPS; Mouse brain microvascular endothelial cells; Sepsis; miR-126a-5p.

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