2. Academic Validation
  3. Design and Synthesis of 7-Oxabicyclo[2.2.1]heptane-2,3-dicarboxylic Acid Derivatives as PP5 Inhibitors To Reverse Temozolomide Resistance in Glioblastoma Multiforme

Design and Synthesis of 7-Oxabicyclo[2.2.1]heptane-2,3-dicarboxylic Acid Derivatives as PP5 Inhibitors To Reverse Temozolomide Resistance in Glioblastoma Multiforme

  • J Med Chem. 2024 Sep 12;67(17):15691-15710. doi: 10.1021/acs.jmedchem.4c01304.
Zekun Li 1 2,  Mochen Guo 1 2,  Mingxiao Gu 1 2,  Zhongtian Cai 1 2,  Qiuyu Wu 1 2,  Jia Yu 1 2,  Meilun Tang 1 2,  Chenxi He 1 2,  Yuxuan Wang 1 2,  Piaoyang Sun 3,  Qidong You 1 2,  Lei Wang 1 2
Affiliations

Affiliations

  • 1 State Key Laboratory of Natural Medicines and Jiangsu Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing 210009, China.
  • 2 Department of Medicinal Chemistry, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
  • 3 Shanghai Hengrui Pharmaceutical Co., Ltd., Shanghai 200245, China.
PMID: 39136241 DOI: 10.1021/acs.jmedchem.4c01304
Abstract

The serine/threonine Phosphatase family is important in tumor progression and survival. Due to the high conserved catalytic domain, designing selective inhibitors is challenging. Herein, we obtained compound 28a with 38-fold enhanced PP5 selectivity (PP2A/5 IC50 = 33.8/0.9 μM) and improved drug-like properties (favorable stability and safety, F = 82.0%) by rational drug design based on a phase II PP2A/5 dual target inhibitor LB-100. Importantly, we found the spatial conformational restriction of the 28a indole fragment was responsible for the selectivity of PP5. Thus, 28a activated p53 and downregulated cyclin D1 and MGMT, which showed potency in cell cycle arrest and reverse temozolomide (TMZ) resistance in the U87 MG cell line. Furthermore, oral administration of 28a and TMZ was well tolerated to effectively inhibit tumor growth (TGI = 87.7%) in the xenograft model. Collectively, these results implicate 28a could be a drug candidate by reversing TMZ resistance with a selective PP5 inhibition manner.

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