2. Academic Validation
  3. Auranofin Suppresses the Growth of Canine Mammary Tumour Cells and Induces Apoptosis via the PI3K/AKT Pathway

Auranofin Suppresses the Growth of Canine Mammary Tumour Cells and Induces Apoptosis via the PI3K/AKT Pathway

  • Vet Comp Oncol. 2024 Sep 2. doi: 10.1111/vco.13005.
Zhaoyan Lin 1 2,  Rong Chen 1 2,  Jiao Wang 1 2,  Yu Zheng 1 2,  Zixuan He 1 2,  Ye Yan 1 2,  Linxi Zhang 1,  Xiaohong Huang 1 2,  Hong Zhang 3
Affiliations

Affiliations

  • 1 College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, China.
  • 2 Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fujian Agriculture and Forestry University, Fuzhou, China.
  • 3 College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
PMID: 39221701 DOI: 10.1111/vco.13005
Abstract

Canine mammary gland tumour (CMT) is the most common spontaneous tumour in intact female dogs and often exhibits metastases. Auranofin (AF) is a gold complex used for treating rheumatism. The excellent anti-tumour ability of AF has been demonstrated in various types of human and canine tumours. In this study, five CMT cell lines (CIPp, CMT-7364, CHMp, CIPm and CTBp) and three CMT primary cells (G7894, L1883 and L6783) were used to explore the anti-tumour effect of AF on CMT. Two CMT cell lines (CIPp and CMT-7364) were used to search the underlying mechanism of the effect of AF on CMT. The results showed that AF inhibited the growth, migration, invasion, and colony formation abilities of CMT cells. Additionally, the growth of CMT in a 3D Cell Culture model was effectively suppressed by AF. Furthermore, AF induced cell Apoptosis of CMT cells via the PI3K/Akt pathway. In conclusion, AF effectively induces CMT Apoptosis by regulating the PI3K/Akt pathway, indicating that AF should be explored as a potential CMT treatment in future studies.

Keywords

3D cell culture; CMT; PI3K/AKT; apoptosis; auranofin.

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