2. Academic Validation
  3. Osteogenic-Like Microenvironment of Renal Interstitium Induced by Osteomodulin Contributes to Randall's Plaque Formation

Osteogenic-Like Microenvironment of Renal Interstitium Induced by Osteomodulin Contributes to Randall's Plaque Formation

  • Adv Sci (Weinh). 2024 Oct;11(40):e2405875. doi: 10.1002/advs.202405875.
Zewu Zhu 1 2 3,  Fang Huang 1 2,  Meng Gao 1 2,  Minghui Liu 1 2,  Youjie Zhang 1 2,  Liang Tang 1 2,  Jian Wu 1 2,  Hao Yu 1 2,  Cheng He 1 2,  Jinbo Chen 1 2,  Zhongqing Yang 1,  Zhiyong Chen 1,  Yang Li 1,  Hequn Chen 1,  Ting Lei 2 4 5,  Feng Zeng 1,  Yu Cui 1
Affiliations

Affiliations

  • 1 Department of Urology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.
  • 2 National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.
  • 3 Department of Internal Medicine, Section Endocrinology, Yale University School of Medicine, New Haven, CT, 06519, USA.
  • 4 Department of Orthopedics, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.
  • 5 Department of Orthopaedic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310006, China.
PMID: 39225583 DOI: 10.1002/advs.202405875
Abstract

Calcium oxalate (CaOx) kidney stones are common and recurrent, lacking pharmacological prevention. Randall's plaques (RPs), calcium deposits in renal papillae, serve as niduses for some CaOx stones. This study explores the role of osteogenic-like cells in RP formation resembling ossification. CaP crystals deposit around renal tubules, interstitium, and blood vessels in RP tissues. Human renal interstitial fibroblasts (hRIFs) exhibit the highest osteogenic-like differentiation potential compared to chloride voltage-gated channel Ka positive tubular epithelial cells, Aquaporin 2 positive collecting duct cells, and vascular endothelial cells, echoing the upregulated osteogenic markers primarily in hRIFs within RP tissues. Utilizing RNA-seq, osteomodulin (OMD) is found to be upregulated in hRIFs within RP tissues and hRIFs following osteogenic induction. Furthermore, OMD colocalizes with CaP crystals and calcium vesicles within RP tissues. OMD can enhance osteogenic-like differentiation of hRIFs in vitro and in vivo. Additionally, crystal deposits are attenuated in mice with Omd deletion in renal interstitial fibroblasts following CaOx nephrocalcinosis induction. Mechanically, a positive feedback loop of OMD/BMP2/BMPR1A/RUNX2/OMD drives hRIFs to adopt osteogenic-like fates, by which OMD induces osteogenic-like microenvironment of renal interstitium to participate in RP formation. We identify OMD upregulation as a pathological feature of RP, paving the way for preventing CaOx stones.

Keywords

Randall's plaques; biomineralization; fibroblasts; kidney stones; osteomodulin.

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