2. Academic Validation
  3. Critical role of apoptosis in MeCP2-mediated epithelial-mesenchymal transition of ARPE-19 cells

Critical role of apoptosis in MeCP2-mediated epithelial-mesenchymal transition of ARPE-19 cells

  • J Cell Physiol. 2024 Sep 5:e31429. doi: 10.1002/jcp.31429.
Yongya Zhang 1 2 3,  Fei Wu 1 2 3,  Sibei Guo 2 3 4,  Ruijie Yin 2 3,  Min Yuan 2 3,  Xue Li 2 3,  Xueru Zhao 2 3,  Xiaohua Li 1 2 3 4 5
Affiliations

Affiliations

  • 1 People's Hospital of Zhengzhou University, Zhengzhou, China.
  • 2 Henan Key Laboratory of Ophthalmology and Visual Science, Henan Eye Institute, Henan Provincial People's Hospital, Henan Eye Hospital, Zhengzhou, China.
  • 3 Eye Institute, Henan Academy of Innovations in Medical Science, Zhengzhou, China.
  • 4 Department of Ophthalmology, People's Hospital of Xinxiang Medical University, Zhengzhou, China.
  • 5 Department of Ophthalmology, People's Hospital of Henan University, Zhengzhou, China.
PMID: 39238182 DOI: 10.1002/jcp.31429
Abstract

Proliferative vitreoretinopathy (PVR) is a complex disease that significantly contributes to recurrent retinal detachment. Its development is notably affected by epithelial-mesenchymal transition (EMT), where Apoptosis plays a crucial role as a regulator of EMT. However, the function of MeCP2 in governing Apoptosis and EMT in retinal pigment epithelial (RPE) cells and its implications for PVR development have remained inadequately understood. Thus, we investigated the impact of MeCP2 on proliferation, migration, Apoptosis and EMT in ARPE-19 cells to provide a fresh perspective on the etiology of PVR. The morphological changes in ARPE-19 cells induced by recombinant human MeCP2 protein and MeCP2 knockdown were observed. Wound healing assay were performed to verify the effects of recombinant human MeCP2 protein and MeCP2 knockdown on ARPE-19 cell migration. Furthermore, cell proliferation was assessed using the CCK-8 assay and flow cytometry. Western blot analysis, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), and immunofluorescence analysis were conducted to measure the protein levels associated with Apoptosis, cell cycle and EMT. Western blot analysis and immunofluorescence assays confirmed that MeCP2 promoted EMT formation in ARPE-19 cells. The CCK-8 assay revealed that MeCP2 treatment enhanced the proliferation of ARPE-19 cells, whereas MeCP2 knockdown inhibited ARPE-19 cell proliferation. Treatment with recombinant human MeCP2 protein and MeCP2 knockdown altered the morphology of ARPE-19 cells. Wound healing assay demonstrated that MeCP2 knockdown inhibited ARPE-19 cell migration, and MeCP2 treatment promoted ARPE-19 cell migration. MeCP2 knockdown induced a G0/G1 phase block, inhibiting cell growth, and qRT-PCR data indicated reduced expression of cell cycle-related genes. Increased Apoptosis was observed after MeCP2 knockdown in ARPE-19 cells. Overall, MeCP2 treatment stimulates cell proliferation, migration and EMT formation; conversely, MeCP2 knockdown inhibits EMT, cell proliferation, migration and cell cycle G1/S phase transition, and induces Apoptosis.

Keywords

EMT; MeCP2; PVR; RPE; apoptosis.

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