2. Academic Validation
  3. Fusobacterium nucleatum extracellular vesicles are enriched in colorectal cancer and facilitate bacterial adhesion

Fusobacterium nucleatum extracellular vesicles are enriched in colorectal cancer and facilitate bacterial adhesion

  • Sci Adv. 2024 Sep 20;10(38):eado0016. doi: 10.1126/sciadv.ado0016.
Xin Zheng 1 2,  Tao Gong 1,  Wanyi Luo 1 2,  Bing Hu 3,  Jinhang Gao 4,  Yuqing Li 1,  Rui Liu 1,  Na Xie 5,  Wenming Yang 6,  Xin Xu 1 2,  Lei Cheng 1 2,  Chenchen Zhou 1,  Quan Yuan 1,  Canhua Huang 5,  Xian Peng 1,  Xuedong Zhou 1 2
Affiliations

Affiliations

  • 1 State Key Laboratory of Oral Diseases, National Center for Stomatology, and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, P.R. China.
  • 2 Department of Cardiology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, P.R. China.
  • 3 Department of Gastroenterology and Hepatology, West China Hospital, Sichuan University, Chengdu 610041, P.R. China.
  • 4 Laboratory of Gastroenterology and Hepatology, West China Hospital, Sichuan University, Chengdu 610041, P.R. China.
  • 5 State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China School of Basic Medical Sciences & Forensic Medicine, and Collaborative Innovation Center for Biotherapy, Sichuan University, Chengdu 610041, P.R. China.
  • 6 Division of Gastrointestinal Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, P.R. China.
PMID: 39303027 DOI: 10.1126/sciadv.ado0016
Abstract

Fusobacterium nucleatum in colorectal Cancer (CRC) tissue is implicated at multiple stages of the disease, while the mechanisms underlying Bacterial translocation and colonization remain incompletely understood. Herein, we investigated whether extracellular vesicles derived from F. nucleatum (FnEVs) have impacts on Bacterial colonization. In mice with colitis-related CRC, a notable enrichment of FnEVs was observed, leading to a significant increase in intratumor colonization by F. nucleatum and accelerated progression of CRC. The enrichment of FnEVs in clinical CRC tissues was demonstrated. Subsequently, we revealed that FnEVs undergo membrane fusion with CRC cells, leading to the transfer and retention of FomA on recipient cell surfaces. Given its ability to facilitate F. nucleatum autoaggregation through interaction with FN1441, the presence of FomA on CRC cell surfaces presents a target for Bacterial adhesion. Collectively, the findings unveil a mechanism used by EVs to prepare a niche conducive for Bacterial colonization in distal organs.

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