2. Academic Validation
  3. Natural small molecule compounds targeting Wnt signaling pathway inhibit HPV infection

Natural small molecule compounds targeting Wnt signaling pathway inhibit HPV infection

  • Microb Pathog. 2024 Nov:196:106960. doi: 10.1016/j.micpath.2024.106960.
Tao Zhang 1,  Ze Wang 2,  Munawaer Muaibati 2,  Fanwei Huang 2,  Kexin Li 2,  Abuduyilimu Abasi 2,  Qing Tong 2,  Dan Wang 3,  Lei Jin 4,  Xiaoyuan Huang 2,  Liang Zhuang 5
Affiliations

Affiliations

  • 1 Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and Technology, 1095 JieFang Avenue, Wuhan, 430030, China; Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen, 518057, China.
  • 2 Department of Obstetrics and Gynecology, Cancer Biology Research Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and Technology, 1095 JieFang Avenue, Wuhan, 430030, China.
  • 3 Department of Ophthalmology, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
  • 4 Reproductive Medicine Center, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and Technology, 1095 JieFang Avenue, Wuhan, 430030, China.
  • 5 Department of Oncology, Tongji Hospital, Tongji Medicine College, Huazhong University of Science and Technology, 1095 JieFang Avenue, Wuhan, 430030, China. Electronic address: zhuangliang@tjh.tjmu.edu.cn.
PMID: 39313132 DOI: 10.1016/j.micpath.2024.106960
Abstract

Background: High-risk human papillomavirus (HPV) Infection is a major risk factor of HPV-related tumors, especially cervical Cancer. To date, there is no specific drug for the treatment of HPV Infection.

Purpose: To explore the role of canonical Wnt signaling pathway in HPV16 Infection and to screen inhibitors against HPV16 Infection from natural small molecule compounds targeting the canonicalWnt pathway.

Methods: Wnt pathway inhibitor IWP-2 and FH535 were used to inhibit Wnt/β-catenin signaling pathway. HPV16-GFP pseudovirus infectivity were analyzed by fluorescence microscopy and fluorescence activated cell sorting. A small molecule screening of a total of CFDA-approved 29 natural compounds targeting the Wnt pathway was performed.

Results: Wnt signaling pathway inhibitor suppressed HPV16-GFP pseudovirus Infection in HaCat cells. Natural small molecule compounds screening identified 6-Gingerol, gossypol, tanshinone II2A, and EGCG as inhibitors of HPV16-GFP pseudovirus Infection.

Conclusion: Wnt signaling pathway is involved in the process of HPV Infection of host cells. 6-Gingerol, gossypol, tanshinone II2A, and EGCG inhibited HPV16-GFP pseudovirus Infection and suppressed Wnt/β-catenin pathway in HaCat cells.

Keywords

6-Gingerol; EGCG; Gossypol; Human papillomavirus; Tanshinone II2A; Wnt/β-catenin signaling pathway.

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