1. Academic Validation
  2. Diosmetin Promotes Early Embryonic Development in Pigs by Alleviating Oxidative Stress

Diosmetin Promotes Early Embryonic Development in Pigs by Alleviating Oxidative Stress

  • Mol Reprod Dev. 2024 Sep;91(9):e23775. doi: 10.1002/mrd.23775.
Jia-Jun Ren 1 Xiu-Wen Yuan 1 Zi-Long Meng 1 Neng-Hao Cao 1 Yong-Nan Xu 1 Nam-Hyung Kim 1 Ying-Hua Li 1
Affiliations

Affiliation

  • 1 Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, South China Institute of Large Animal Models for Biomedicine, Wuyi University, Jiangmen, China.
Abstract

Diosmetin (DIOS), a natural flavonoid monomer derived from lemons and present in various Plants such as spearmint and spider moss, exhibits antioxidant, anti-inflammatory, and antiaging properties. Nonetheless, its impact on early embryonic development in pigs remains unexplored. This study aimed to determine the influence of DIOS supplementation in an in vitro culture (IVC) medium on porcine embryo development and to elucidate the underlying mechanisms. Findings revealed that embryos cultured in IVC medium with 0.1 μM DIOS demonstrated an increased blastocyst formation rate, higher total cell number, reduced LC3B and CASPASE3 levels, elevated Nrf2 levels, decreased ROS, and enhanced GSH and mitochondrial membrane potential at the 4-cell embryonic stage. Additionally, the expression of proapoptotic genes (CAS3, CAS8, and Bax) and autophagy-related genes (Beclin1, ATG5, LC3B, and p62) was downregulated, whereas the expression of embryonic development-related genes (CDK1 and CDK2), antioxidant-related genes (SOD1 and SOD2), and mitochondrial biogenesis-related genes (NRF2) was upregulated. These findings suggest that DIOS promotes early embryonic development in pigs by mitigating oxidative stress and enhancing mitochondrial function, thereby reducing Autophagy and Apoptosis levels.

Keywords

apoptosis; autophagy; diosmetin; flavonoid; porcine embryo.

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