2. Academic Validation
  3. Infected wound repair correlates with collagen I induction and NOX2 activation by cold atmospheric plasma

Infected wound repair correlates with collagen I induction and NOX2 activation by cold atmospheric plasma

  • NPJ Regen Med. 2024 Oct 2;9(1):28. doi: 10.1038/s41536-024-00372-0.
Océane Blaise # 1 2,  Constance Duchesne # 1 2,  Elena Capuzzo # 1,  Marie-Anne Nahori 3,  Julien Fernandes 4,  Michael G Connor 5,  Mélanie A Hamon 5,  Javier Pizarro-Cerda 1,  Jean-Jacques Lataillade 6,  Colin McGuckin 7,  Antoine Rousseau 2,  Sébastien Banzet 6 8,  Olivier Dussurget 9,  Nadira Frescaline 10 11
Affiliations

Affiliations

  • 1 Institut Pasteur, Université Paris Cité, CNRS UMR6047, Unité de Recherche Yersinia, Paris, France.
  • 2 École Polytechnique, Sorbonne Université, CNRS UMR7648, Laboratoire de Physique des Plasmas, Palaiseau, France.
  • 3 Institut Pasteur, Université Paris Cité, CNRS UMR6047, Unité des Toxines Bactériennes, Paris, France.
  • 4 Institut Pasteur, UTechS PBI, C2RT, Paris, France.
  • 5 Institut Pasteur, Université Paris Cité, Unité Chromatine et Infection, Paris, France.
  • 6 Centre de Transfusion Sanguine des Armées, Clamart, France.
  • 7 CTI Biotech, Meyzieu, France.
  • 8 Institut de Recherche Biomédicale des Armées, INSERM UMRS-MD 1197, Brétigny-sur-Orge, France.
  • 9 Institut Pasteur, Université Paris Cité, CNRS UMR6047, Unité de Recherche Yersinia, Paris, France. olivier.dussurget@pasteur.fr.
  • 10 Institut Pasteur, Université Paris Cité, CNRS UMR6047, Unité de Recherche Yersinia, Paris, France. nadira.frescaline@intradef.gouv.fr.
  • 11 Centre de Transfusion Sanguine des Armées, Clamart, France. nadira.frescaline@intradef.gouv.fr.
  • # Contributed equally.
PMID: 39358383 DOI: 10.1038/s41536-024-00372-0
Abstract

Cold atmospheric plasma (CAP) is a promising complement to tissue repair and regenerative medicine approaches. CAP has therapeutic potential in infected cutaneous wounds by mechanisms which remain enigmatic. Here, CAP is shown to activate phagocyte NADPH Oxidase complex NOX2. CAP induced increased intracellular Reactive Oxygen Species, alleviated by NOX2 inhibitors. Genetic and pharmacological inhibitions of NOX2 in macrophages and bioengineered skin infected with Staphylococcus aureus and treated with CAP reduced intracellular oxidants and increased Bacterial survival. CAP triggered Rac activation and phosphorylation of p40phox and p47phox required for NOX2 assembly and activity. Furthermore, CAP induced collagen I expression by fibroblasts. Infection and healing kinetics showed that murine skin wounds infected with S. aureus and treated with CAP are characterized by decreased Bacterial burden, increased length of neoepidermis and extracellular matrix formation. Collectively, our findings identify mechanisms triggered by CAP that subdue Infection and result in enhanced repair following skin injury.

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