2. Academic Validation
  3. Development of a TBXT-EGFP iPS cell model for screening the early developmental toxicity of typical environmental pollutants

Development of a TBXT-EGFP iPS cell model for screening the early developmental toxicity of typical environmental pollutants

  • Food Chem Toxicol. 2024 Nov:193:115039. doi: 10.1016/j.fct.2024.115039.
Yanyi Zhao 1,  Miaomiao Zhao 2,  Qingyuan Li 1,  Hanyue Li 1,  Renjun Yang 1,  Nuoya Yin 3,  Francesco Faiola 1
Affiliations

Affiliations

  • 1 State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 100049, China.
  • 2 State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 100049, China; Institute of Life Science and Green Development/College of Life Sciences, Hebei University, Baoding, 071002, China.
  • 3 State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address: nyyin@rcees.ac.cn.
PMID: 39389444 DOI: 10.1016/j.fct.2024.115039
Abstract

In our daily lives, we are inevitably exposed to a variety of environmental pollutants in numerous ways. Fortunately, recent years have witnessed significant advancements in the field of stem cell toxicology, which have provided new opportunities for research in environmental toxicology. Applying stem cell technology to environmental toxicology, overcomes some of the limitations of traditional screening methods and we can more accurately predict the toxicity of environmental pollutants. However, there are still several aspects of stem cell toxicology models that require improvement, such as increasing the throughput of detection and simplifying detection methods. Consequently, we developed an environmental pollutant toxicity detection model based on TBXT-EGFP iPS cells and screened the developmental toxicity of 38 typical environmental pollutants. Our results indicate that TBBPA-BDBPE, TBBPA-BHEE, DG, and AO2246 may interfere with the expression of TBXT, a critical marker gene for early human embryo development, implying that these environmental pollutants could lead to developmental abnormalities.

Keywords

Developmental toxicity; Environmental pollutant; Stem cell toxicology; TBXT-EGFP induced pluripotent stem (iPS) cells.

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