Pterostilbene Induces Pyroptosis in Breast Cancer Cells through Pyruvate Kinase 2/Caspase-8/Gasdermin C Signaling Pathway

The incidence and mortality of breast Cancer increase year by year, and it is urgent to find high-efficiency and low-toxicity anti-cancer drugs. Pterostilbene (PTE) is a natural product with antitumor activity, but the specific antitumor mechanism is not very clear. Aerobic glycolysis is the main energy supply for Cancer cells. Pyroptosis is an inflammatory, programmed cell death. The aim of this study was to investigate the effect of PTE on glycolysis and Pyroptosis in EMT6 and 4T1 cells and the specific mechanism, and to elucidate the role of Pyruvate Kinase 2 (PKM2), a key Enzyme in glycolysis, in the antitumor role of PTE. Our study suggested that PTE induced Pyroptosis by inhibiting tumor glycolysis. PKM2 played an important role in both the inhibition of glycolysis and the induction of Pyroptosis by PTE.

GSDMC; PKM2; Pterostilbene; breast cancer; caspase-8; metabolism; pyroptosis.