2. Academic Validation
  3. Corilagin inhibits human cytomegalovirus infection and replication via activating the cGAS-STING signaling pathway in vitro and in vivo

Corilagin inhibits human cytomegalovirus infection and replication via activating the cGAS-STING signaling pathway in vitro and in vivo

  • Int Immunopharmacol. 2024 Dec 25;143(Pt 2):113401. doi: 10.1016/j.intimp.2024.113401.
Jiao Xie 1,  Luorui Shang 2,  Cuiling Liu 3,  Jinqian Mao 4,  Chengyi He 5,  Miao Luo 6,  David Fisher 7,  Nguyen Thi Thu Hien 8,  Sanping Xu 9,  Lei Zhao 10
Affiliations

Affiliations

  • 1 Health Management Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
  • 2 Department of Integrated Traditional Chinese and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
  • 3 The Tenth Affiliated Hospital of Southern Medical University, Dongguan, China.
  • 4 Department of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
  • 5 Department of Vascular Surgery, the Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, China.
  • 6 Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
  • 7 Department of Medical Biosciences, Faculty of Natural Sciences, University of The Western Cape, Cape Town, South Africa.
  • 8 Hai Phong University of Medicine and Pharmacy, Hai Phong, Viet Nam.
  • 9 Health Management Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address: xusanpinghao@aliyun.com.
  • 10 Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Electronic address: laizhao@hust.edu.cn.
PMID: 39423664 DOI: 10.1016/j.intimp.2024.113401
Abstract

Aim: The existence of human cytomegalovirus (HCMV) is extremely widespread, causing serious diseases in patients with low immune function. The purpose of this study is to explore the efficacy and mechanism of Corilagin in the control of CMV Infection, in order to provide scientific basis for the control of CMV Infection.

Methods: Our study employed an animal model in Balb/c mice, infected with MCMV, alongside cellular models in HFF cells and THP-1 cells, stimulated with HCMV. The expression of cGAS-STING signaling pathway molecules was detected in liver tissue, lung tissue, serum, cells and cell supernatant. The liver function and histopathological changes of mice were evaluated.

Results: In vivo and in vitro experiments showed that Corilagin significantly inhibits CMV levels and attenuates pathological damage in liver and lung tissues in vivo, and similarly inhibits viral load in cells in vitro. Corilagin promotes the expression levels of STING and its downstream molecules in vivo and in vitro. Inhibition/down-regulation of STING significantly promotes CMV replication, on the contrary, activation/up-regulation of STING inhibits CMV replication, and Corilagin also promotes the expression levels of molecules related to the cGAS-STING signaling pathway in the above cases.

Conclusion: Corilagin could effectively inhibit the Infection and replication of CMV in vitro and in vivo, which may be through the activation of cGAS-STING signaling pathway.

Keywords

Antivirus; Corilagin; Cytomegalovirus; STING; cGAS.

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