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  3. Size-reduced DREADD derivatives for AAV-assisted multimodal chemogenetic control of neuronal activity and behavior

Size-reduced DREADD derivatives for AAV-assisted multimodal chemogenetic control of neuronal activity and behavior

  • Cell Rep Methods. 2024 Oct 21;4(10):100881. doi: 10.1016/j.crmeth.2024.100881.
Takahito Miyake 1,  Kaho Tanaka 2,  Yutsuki Inoue 2,  Yuji Nagai 3,  Reo Nishimura 2,  Takehito Seta 2,  Shumpei Nakagawa 2,  Ken-Ichi Inoue 4,  Emi Hasegawa 2,  Takafumi Minamimoto 3,  Masao Doi 5
Affiliations

Affiliations

  • 1 Department of Systems Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyō-ku, Kyoto 606-8501, Japan. Electronic address: tmiyake@pharm.kyoto-u.ac.jp.
  • 2 Department of Systems Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyō-ku, Kyoto 606-8501, Japan.
  • 3 Advanced Neuroimaging Center, National Institutes for Quantum Science and Technology, Chiba 263-8555, Japan.
  • 4 Systems Neuroscience Section, Center for the Evolutionary Origins of Human Behavior, Kyoto University, Inuyama 484-8506, Japan.
  • 5 Department of Systems Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyō-ku, Kyoto 606-8501, Japan. Electronic address: doimasao@pharm.kyoto-u.ac.jp.
PMID: 39437713 DOI: 10.1016/j.crmeth.2024.100881
Abstract

Designer receptors exclusively activated by designer drugs (DREADDs) are engineered G-protein-coupled receptors that afford reversible manipulation of neuronal activity in vivo. Here, we introduce size-reduced DREADD derivatives miniDq and miniDi, which inherit the basic receptor properties from the Gq-coupled excitatory receptor hM3Dq and the Gi-coupled inhibitory receptor hM4Di, respectively, while being approximately 30% smaller in size. Taking advantage of the compact size of the receptors, we generated an adeno-associated virus (AAV) vector carrying both miniDq and the Other DREADD family receptor (κ-opioid receptor-based inhibitory DREADD [KORD]) within the maximum AAV capacity (4.7 kb), allowing us to modulate neuronal activity and animal behavior in both excitatory and inhibitory directions using a single viral vector. We confirmed that expressing miniDq, but not miniDi, allowed activation of striatum activity in the cynomolgus monkey (Macaca fascicularis). The compact DREADDs may thus widen the opportunity for multiplexed interrogation and/or intervention in neuronal regulation in mice and non-human primates.

Keywords

AAV; CP: Neuroscience; DREADD; bidirectional manipulation; body temperature; hM3D(q); hM4D(i); non-human primate.

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