2. Academic Validation
  3. Potential antiviral activity of rhamnocitrin against influenza virus H3N2 by inhibiting cGAS/STING pathway in vitro

Potential antiviral activity of rhamnocitrin against influenza virus H3N2 by inhibiting cGAS/STING pathway in vitro

  • Sci Rep. 2024 Nov 16;14(1):28287. doi: 10.1038/s41598-024-79788-z.
Zexing Chen # 1 2,  Wanqi Wang # 1 2,  Kefeng Zeng 2,  Jinyi Zhu 1 2,  Xinhua Wang 1 2,  Wanyi Huang 3 4
Affiliations

Affiliations

  • 1 State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510180, China.
  • 2 Institute of Integration of Traditional and Western Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
  • 3 State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510180, China. 13724161240@163.com.
  • 4 Institute of Integration of Traditional and Western Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. 13724161240@163.com.
  • # Contributed equally.
PMID: 39550441 DOI: 10.1038/s41598-024-79788-z
Abstract

Influenza remains a serious issue for public health and it's urgent to discover more effected drugs against Influenza Virus. Rhamnocitrin, as a flavonoid, its effect on Influenza Virus infection remains poorly explored. In this study, rhamnocitrin showed Antiviral effect and anti-apoptosis on Influenza Virus A/Aichi/2/1968 (H3N2) in MDCK cells and A549 cells. In addition, molecular docking revealed that rhamnocitrin have good binding activity with the target proteins cGAS and STING, molecular dynamic simulation and surface plasmon resonance showed that rhamnocitrin could form a stable complex with the above proteins. Moreover, the qPCR and western blot assays further verified that rhamnocitrin could reduce type I IFN and proinflammatory cytokines production by inhibiting the cGAS/STING pathway. Taken together, the results suggest that rhamnocitrin could be a potential anti-viral agent against influenza.

Keywords

Anti-viral; Apoptosis; Influenza; Rhamnocitrin; STING; cGAS.

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