2. Academic Validation
  3. Protocol for cryopreserving neural organoids and human living brain tissue using MEDY

Protocol for cryopreserving neural organoids and human living brain tissue using MEDY

  • STAR Protoc. 2024 Dec 20;5(4):103466. doi: 10.1016/j.xpro.2024.103466.
Weiwei Xue 1,  Jiacheng Du 2,  Yunteng Li 2,  Zhicheng Shao 3
Affiliations

Affiliations

  • 1 Jinshan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institute of Pediatrics, National Children's Medical Center, Children's Hospital, Fudan University, Shanghai 200032, China. Electronic address: wwxue@fudan.edu.cn.
  • 2 Jinshan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institute of Pediatrics, National Children's Medical Center, Children's Hospital, Fudan University, Shanghai 200032, China.
  • 3 Jinshan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institute of Pediatrics, National Children's Medical Center, Children's Hospital, Fudan University, Shanghai 200032, China; Department of Neurology, Zhongshan Hospital, Institute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Fudan University, Shanghai 200032, China. Electronic address: zcshao@fudan.edu.cn.
PMID: 39607824 DOI: 10.1016/j.xpro.2024.103466
Abstract

Cryopreservation of neural organoids and human living brain tissue faces huge challenges. Here, we present a cryopreservation protocol for the reliable storage of diverse neural organoids and human living brain tissue using methylcellulose, ethylene glycol, DMSO, and Y27632, termed MEDY. We describe steps for preparing cryopreservation solution, preparing cortical organoids, and surgical removal of human brain tissue. We then detail procedures for cryopreservation, resuscitation, and detection assays. This protocol can serve as a model for studying human neurological diseases. For complete details on the use and execution of this protocol, please refer to Xue et al.1.

Keywords

neuroscience; organoids; stem cells.

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