2. Academic Validation
  3. Neuroligin 1 Regulates Autistic-Like Repetitive Behavior through Modulating the Activity of Striatal D2 Receptor-Expressing Medium Spiny Neurons

Neuroligin 1 Regulates Autistic-Like Repetitive Behavior through Modulating the Activity of Striatal D2 Receptor-Expressing Medium Spiny Neurons

  • Adv Sci (Weinh). 2025 Feb;12(5):e2410728. doi: 10.1002/advs.202410728.
Dandan Lv 1 2,  An Liu 1 2 3,  Ziyue Yi 1 2,  Mingdao Mu 1 4,  Miao Wu 1 2,  Xingcan Li 1 2,  Kun Cao 1 2,  Ruining Liu 1 2,  Zhengping Jia 5 6,  Junhai Han 1 2,  Wei Xie 1 2 7
Affiliations

Affiliations

  • 1 The Key Laboratory of Developmental Genes and Human Disease, The School of Life Science and Technology, Southeast University, 2 Sipailou Road, Nanjing, 210096, China.
  • 2 Institute for Brain and Intelligence, Southeast University, 2 Sipailou Road, Nanjing, 210096, China.
  • 3 Shenzhen Research Institute, Southeast University, 19 Gaoxin South 4th Road, Shenzhen, 518063, China.
  • 4 School of Medicine, Southeast University, 87 Dingjiaqiao Road, Nanjing, 210009, China.
  • 5 Neurosciences & Mental Health, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario, M5G 1×8, Canada.
  • 6 Department of Physiology, Faculty of Medicine, University of Toronto, 1 King's College Circle, Toronto, Ontario, M5S 1A8, Canada.
  • 7 Jiangsu Co-innovation Center of Neuroregeneration, Southeast University, 2 Sipailou Road, Nanjing, 210096, China.
PMID: 39661696 DOI: 10.1002/advs.202410728
Abstract

Restricted and repetitive behavior (RRB) is a primary symptom of autism spectrum disorder (ASD), which poses a significant risk to individuals' health and is becoming increasingly prevalent. However, the specific cellular and neural circuit mechanisms underlying the generation of RRB remain unclear. In this study, it is reported that the absence of the ASD-related protein Neuroligin 1 (NLGN1) in Dopamine Receptor D2-expressing medium spiny neurons (D2-MSNs) in the dorsal striatum is associated with the duration and frequency of self-grooming and digging behaviors. The Nlgn1-deficient D2-MSNs are hyperactivated, which correlates with excessive self-grooming and digging behaviors. Inhibiting the activity of D2-MSNs reduces the duration and frequency of these RRBs. Furthermore, it is demonstrated that the generation of self-grooming and digging behaviors depends on distinct patterns of D2-MSN activity. Finally, through single-nucleus RNA Sequencing (sn-RNAseq) and protein detection verification, it is revealed that the overactivation of protein kinase C (PKC) in Nlgn1-deficient mice contributes to excessive repetitive behaviors and increased neuronal excitability. In this study, potential mechanisms are proposed for the generation of self-grooming and digging behaviors, as well as suggest possible treatments and interventions ASD.

Keywords

Autism Spectrum Disorder (ASD); D2‐MSN; neuroligin1; restricted and repetitive behavior (RRB); striatum.

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