2. Academic Validation
  3. An active protein from Dendrobium officinale residue: Protects the gastric mucosa and stabilized in the gastrointestinal tract

An active protein from Dendrobium officinale residue: Protects the gastric mucosa and stabilized in the gastrointestinal tract

  • Int J Biol Macromol. 2025 Mar:294:139387. doi: 10.1016/j.ijbiomac.2024.139387.
Daian Pan 1,  Yanling Sun 2,  Jiayi Zhang 2,  Jing Zeng 2,  Shiting Yu 2,  Daqing Zhao 2,  Zhengqi Dong 3,  Meichen Liu 2,  Shichao Liu 4,  Weinan Wang 5,  Siming Wang 6
Affiliations

Affiliations

  • 1 Changchun University of Chinese Medicine, Key Laboratory of Ginseng Efficacy Substance Base and Biological Mechanism Research, Ministry of Education, Changchun 130117, China; Northeast Asia Research Institute of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun 130117, China; Research Center of Traditional Chinese Medicine, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun 130021, China.
  • 2 Changchun University of Chinese Medicine, Key Laboratory of Ginseng Efficacy Substance Base and Biological Mechanism Research, Ministry of Education, Changchun 130117, China; Northeast Asia Research Institute of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun 130117, China.
  • 3 State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Chinese Academy of Medical Sciences & Peking Union Medical College, Institute of Medicinal Plant Development, Beijing 100193, China.
  • 4 Changchun University of Chinese Medicine, Key Laboratory of Ginseng Efficacy Substance Base and Biological Mechanism Research, Ministry of Education, Changchun 130117, China; College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China. Electronic address: aralekyra@163.com.
  • 5 Guangdong Provincial Key Laboratory of Natural Drugs Research and Development, The Affiliated Dongguan Songshan Lake Central Hospital, Guangdong Medical University, Dongguan 523808, China; Northeast Asia Research Institute of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun 130117, China. Electronic address: cnweinanwang@gdmu.edu.cn.
  • 6 Changchun University of Chinese Medicine, Key Laboratory of Ginseng Efficacy Substance Base and Biological Mechanism Research, Ministry of Education, Changchun 130117, China; Northeast Asia Research Institute of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun 130117, China. Electronic address: lwsm126030@126.com.
PMID: 39753178 DOI: 10.1016/j.ijbiomac.2024.139387
Abstract

A large number of by-products generated in the food industry is discarded as waste, especially the residue left after extracting plant resources, which is typically repurposed as fertilizer. In this study, we extracted and purified a new protein, DOP1, from the residue of Dendrobium officinale Kimura & Migo (D. officinale), and explored the protective effect of DOP1 on alcohol-induced gastric mucosal injury. Its amino acid composition and the stability of secondary structure were measured by amino acid analysis and various spectroscopic methods. The microscopic morphology of DOP1 was observed by electron microscopy and its particle distribution was determined to be in the range of 3.7-5.5 nm. In addition, DOP1 was found to exhibit excellent protective activity against alcohol-induced gastric mucosal injury, as well as anti-inflammatory and antioxidant activities by in vitro and in vivo activity assays, which may be related to the upregulation of TFF2 through the activation of PPARγ. Intriguingly, DOP1 does not degrade and remains active after being digested by the gastrointestinal tract. From an economic and sustainable perspective, the discovery of DOP1 will provide new opportunities for the enhanced utilization and sustainable development of D. officinale resources.

Keywords

Dendrobium officinale; Digestive stability; Gastric mucosal protection; Protein; Resource recovery; Structure characterization.

Figures
Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-16578
    99.79%, PPARγ Antagonist