2. Academic Validation
  3. RNA-binding protein DAZAP1 accelerates the advancement of pancreatic cancer by inhibiting ferroptosis

RNA-binding protein DAZAP1 accelerates the advancement of pancreatic cancer by inhibiting ferroptosis

  • Eur J Med Res. 2025 Jan 4;30(1):3. doi: 10.1186/s40001-024-02261-0.
Xinqing Wang 1,  Hao Fan 2,  Xiaoping Ye 1,  Yu Hu 2,  Yan Xiao 2,  Ming Zhang 2,  Yonghui Xu 3,  Jianjun Song 1,  Yongyun Luo 4
Affiliations

Affiliations

  • 1 Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University, Shengli Street, Xingqing District, Ningxia Hui Autonomous Region 804, Yinchuan City, 753400, China.
  • 2 School of Clinical Medicine, Ningxia Medical University, Yinchuan City, China.
  • 3 Department of Pathology, Ningxia Medical University, Yinchuan City, China.
  • 4 Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University, Shengli Street, Xingqing District, Ningxia Hui Autonomous Region 804, Yinchuan City, 753400, China. jack5976@163.com.
PMID: 39754243 DOI: 10.1186/s40001-024-02261-0
Abstract

Background: Pancreatic Cancer (PC) is a highly aggressive malignancy with a poor prognosis due to its late-stage diagnosis and limited treatment options.

Objectives: This study aimed to elucidate the molecular mechanisms underlying PC progression and identify potential molecular targets for its diagnosis and treatment.

Methods: DAZAP1 expression in PC tissues, normal tissues and cell lines was assessed using immunohistochemistry (IHC), reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting. DAZAP1 knockdown was achieved through plasmid transfection, and its effects on Ferroptosis and PC progression were evaluated using RT-qPCR, western blotting, CCK-8 assays, EdU staining, Fe2+ content measurement, Reactive Oxygen Species (ROS) detection, wound healing and Transwell migration assays.

Results: DAZAP1 expression was significantly upregulated in PC tissues and cell lines compared to normal counterparts. DAZAP1 knockdown suppressed PC cell proliferation and induced Ferroptosis, while Ferroptosis inhibition reversed these effects, enhancing PC cell proliferation and metastasis.

Conclusions: DAZAP1 suppression promotes Ferroptosis, thereby inhibiting PC cell proliferation and metastasis. These findings suggest that DAZAP1 is a potential therapeutic target for PC.

Keywords

DAZAP1; Ferroptosis; Metastasis; Pancreatic cancer; Proliferation.

Figures
Products