2. Academic Validation
  3. Neuroprotective potential of isofraxidin: Alleviating parkinsonian symptoms, inflammation and microglial activation

Neuroprotective potential of isofraxidin: Alleviating parkinsonian symptoms, inflammation and microglial activation

  • J Cent Nerv Syst Dis. 2025 Jan 9:17:11795735241312661. doi: 10.1177/11795735241312661.
Tin-An Wang 1 2,  Shiao-Yun Li 3 4,  Li-Yun Fann 5 6,  I-Hsun Li 2 4 7,  Tsung-Ta Liu 2 8,  Hao-Yuan Hung 3 7,  Chieh-Wen Chang 3,  Chih-Chien Cheng 9 10,  Ying-Che Huang 11,  Pei-Yeh Yu 12,  Jui-Hu Shih 2 3 7
Affiliations

Affiliations

  • 1 Graduate Institute of Life Science, National Defense Medical Center, Taipei, Taiwan.
  • 2 School of Pharmacy, National Defense Medical Center, Taipei, Taiwan.
  • 3 Department of Pharmacy Practice, Tri-Service General Hospital, Taipei, Taiwan.
  • 4 Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan.
  • 5 Department of Nursing, Taipei City Hospital, Taipei, Taiwan.
  • 6 Department of Nurse-Midwifery and Women Health, National Taipei University of Nursing and Health Sciences, Taipei, Taiwan.
  • 7 Department of Pharmacology, National Defense Medical Center, Taipei, Taiwan.
  • 8 Department of Biology and Anatomy, National Defense Medical Center, Taipei, Taiwan.
  • 9 Department of Obstetrics/Gynecology, Taipei City Hospital, Taipei, Taiwan.
  • 10 School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan.
  • 11 Department of Anesthesia and Critical Care Medicine, Taipei City Hospital, Taipei, Taiwan.
  • 12 Department of Anesthesiology, Taipei City Hospital, Taipei, Taiwan.
PMID: 39790641 DOI: 10.1177/11795735241312661
Abstract

Background: Parkinson's disease (PD) is one of the most common neurodegenerative disorders. Previous research has confirmed that isofraxidin can reduce macrophage expression and inhibit peripheral inflammation. However, its effects on the central nervous system remain underexplored.

Objective: This study aims to determine whether isofraxidin offers protective effects against PD.

Methods: To assess the effects of isofraxidin, motor performance changes in LPS-induced PD mice were evaluated using rotarod, pole-climbing, and beam-walking tests. Striatal damage was examined through [18F]fluorodeoxyglucose ([18F]FDG) positron emission tomography (PET) imaging, and dopaminergic neurotoxicity was assessed using tyrosine hydroxylase (TH) staining. Microglial accumulation and activation were monitored with Iba-1 staining, while LPS-induced inflammation was examined via TNF-α and IL-1β staining.

Results: Isofraxidin pre-treatment significantly improved LPS-induced motor dysfunction, as evidenced by better performance in the rotarod, pole-climbing, and beam-walking tests. [18F]FDG PET imaging showed that isofraxidin restored glucose uptake in the striatum, countering LPS-induced damage. Furthermore, Iba-1 staining revealed that isofraxidin markedly inhibited LPS-induced microglial activation and accumulation. TNF-α and IL-1β staining indicated a reduction in inflammation with isofraxidin treatment. Additionally, TH staining supported the neuroprotective role of isofraxidin on dopaminergic neurons.

Conclusions: Isofraxidin exhibits notable neuroprotective properties by mitigating LPS-induced parkinsonian behaviors, microglial activation, inflammation, and dopaminergic neuron damage. These results highlight isofraxidin's potential as a therapeutic intervention for PD.

Keywords

Isofraxidin; PET imaging; Parkinson’s disease; TH; inflammation; microglia.

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