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  3. Protocol for inducing beige adipocytes in white adipose tissue of mouse using cold exposure and CL316,243 injection

Protocol for inducing beige adipocytes in white adipose tissue of mouse using cold exposure and CL316,243 injection

  • STAR Protoc. 2025 Jan 14;6(1):103337. doi: 10.1016/j.xpro.2024.103337.
Yalan Wu 1,  Chenguang Wang 2,  Xiao Yu Tian 3
Affiliations

Affiliations

  • 1 School of Biomedical Sciences, Heart and Vascular Institute, The Chinese University of Hong Kong, Hong Kong SAR, China; Department of Histology and Embryology, School of Basic Medical Sciences, Central South University, Changsha 410013, China. Electronic address: wuyalan0327@csu.edu.cn.
  • 2 School of Biomedical Sciences, Heart and Vascular Institute, The Chinese University of Hong Kong, Hong Kong SAR, China; Department of Hematology and Oncology, Shenzhen University General Hospital, Shenzhen University, Shenzhen 518071, China.
  • 3 School of Biomedical Sciences, Heart and Vascular Institute, The Chinese University of Hong Kong, Hong Kong SAR, China. Electronic address: xytian@cuhk.edu.hk.
PMID: 39817914 DOI: 10.1016/j.xpro.2024.103337
Abstract

White adipose tissue (WAT) beiging holds significant therapeutic potential for combating obesity. Here, we present a protocol for inducing beige WAT in mice using both cold exposure and CL316,243 treatment. We describe steps for intraperitoneal injection, and subcutaneous WAT (sWAT) isolation, dissection, and fixation. We then detail procedures for histology, whole-mount immunofluorescence (IF) staining, and extracting RNA and protein. This protocol can be used for subsequent analysis to explore the mechanisms governing beige WAT induction in experimental settings, particularly the evaluation of angiogenesis. For complete details on the use and execution of this protocol, please refer to Wang et al.1.

Keywords

Cell Biology; Metabolism; Model Organisms.

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