2. Academic Validation
  3. Host factor DIAPH1 regulates pseudorabivirus replication by modulating the dynamics of cytoskeleton

Host factor DIAPH1 regulates pseudorabivirus replication by modulating the dynamics of cytoskeleton

  • Int J Biol Macromol. 2025 Apr:298:140112. doi: 10.1016/j.ijbiomac.2025.140112.
Jie-Yuan Guo 1,  Kun Xu 1,  Xiao-Han Wang 1,  Xin-Man Li 1,  Yan-Pei Ku 1,  Lei Zeng 1,  Bo Wan 2,  Guo-Yu Yang 3,  Jiang Wang 4,  Bei-Bei Chu 4,  Jia-Jia Pan 5,  Wen-Bo Hao 6
Affiliations

Affiliations

  • 1 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Zhengzhou 450046, China; Key Laboratory of Veterinary Biotechnology of Henan Province, Zhengzhou 450046, China.
  • 2 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Zhengzhou 450046, China.
  • 3 Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Zhengzhou 450046, China; Key Laboratory of Veterinary Biotechnology of Henan Province, Zhengzhou 450046, China; Henan University of Animal Husbandry and Economy, Zhengzhou 450047, China.
  • 4 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Zhengzhou 450046, China; Key Laboratory of Veterinary Biotechnology of Henan Province, Zhengzhou 450046, China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Zhengzhou 450046, China.
  • 5 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Zhengzhou 450046, China; Key Laboratory of Veterinary Biotechnology of Henan Province, Zhengzhou 450046, China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Zhengzhou 450046, China. Electronic address: first2015@163.com.
  • 6 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China. Electronic address: wbhao@henau.edu.cn.
PMID: 39842589 DOI: 10.1016/j.ijbiomac.2025.140112
Abstract

As obligate parasites, viruses exploit host cell organelles and molecular components to complete their life cycle. Among which, viruses firstly hijack the Cytoskeleton of host cells to ensure their efficiently cell entry and replication. Although formin family members play a key role in both microfilament and microtubule cytoskeletal remodeling, few studies addressed the detailed function and mechanism of formins in the process of viral Infection. Here, we showed that sus scrofa DIAPH1 was involved in the regulation of cytoskeletal dynamics during PRV replication. Firstly, we found that DIAPH1 showed significant changes in the expression level and intracellular localization during PRV Infection of PK-15 cells. Next, inhibition of DIAPH1 by RNA interference or small molecular inhibitor SMIFH2 was found to diminish the outcome of PRV Infection. Besides, DIAPH1 partially co-localized with actin and tubulin in PRV-infected cells. Cross-talk occurred between microfilaments and microfilaments, which also had an influence on the intracellular localization of DIAPH1. What's more, inhibition of DIAPH1 induced the reorganization of microfilament and the stability of microtubule. These results suggested that DIAPH1 regulated PRV Infection by remodeling microfilament and microtubule cytoskeletal dynamics.

Keywords

Actin; Cytoskeleton; DIAPH1; Microtubule; Pseudorabivirus replication.

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