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  3. Glutamate molecular structure and protein affect the inhibition of breast cancer cell metastasis: Cell-derived exosomes inhibitory effects through the MAPK signaling pathway

Glutamate molecular structure and protein affect the inhibition of breast cancer cell metastasis: Cell-derived exosomes inhibitory effects through the MAPK signaling pathway

  • Int J Biol Macromol. 2025 Apr:300:140264. doi: 10.1016/j.ijbiomac.2025.140264.
Yongcheng Chen 1,  Huan Liu 1,  Lang Zhang 1,  Huifang Zeng 1,  LiHe Jiang 2,  Qinghong Qin 3,  Dequan Li 4,  Guanming Lu 5
Affiliations

Affiliations

  • 1 Department of Breast and Thyroid Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, and Key Laboratory of Molecular Pathology in Tumors of Baise, Baise 533000, Guangxi Province, PR China.
  • 2 Xiamen Key Laboratory of Endocrine-Related Cancer Precision Medicine, Xiangan Hospital of Xiamen University, Xiamen 361101, Fujian, PR China.
  • 3 Department of Breast Surgery, Guangxi Medical University Cancer Hospital, 71 Hedi Road, Nanning 530021, Guangxi Province, PR China. Electronic address: qqh00150982@163.com.
  • 4 Department of Breast Surgery, Wuming Hospital of Guangxi Medical University, No.26 Yongning Road, Wuming District, Nanning 530199, Guangxi Province, PR China. Electronic address: lidequan661025@163.com.
  • 5 Department of Breast and Thyroid Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, and Key Laboratory of Molecular Pathology in Tumors of Baise, Baise 533000, Guangxi Province, PR China; Department of Oncology-Pathology, Karolinska Institutet, Stockholm SE-17176, Sweden. Electronic address: luguanming@yeah.net.
PMID: 39863225 DOI: 10.1016/j.ijbiomac.2025.140264
Abstract

The aim of this study was to investigate the inhibitory effect of glutamate molecular structure and protein on breast Cancer cell metastasis and the potential inhibitory mechanism of cell-derived exosomes via MAPK signaling pathway. Breast Cancer cell lines with high metastatic potential were selected by in vitro Cell Culture technique. The effects of specific inhibitors of glutamic acid on the proliferation and metastasis of breast Cancer cells were studied. Changes in protein expression profiles were analyzed by proteomics techniques to identify key proteins associated with breast Cancer metastasis. Breast Cancer cells were treated with inhibitors of the MAPK signaling pathway to evaluate their effect on cell metastasis and compare with exosome treatment. The results showed that the specific inhibitors of glutamate molecular structure could significantly inhibit the proliferation and metastasis of breast Cancer cells. Proteomic analysis revealed several down-regulated proteins that are closely related to breast Cancer metastasis.

Keywords

Breast cancer; Cell metastasis; Exosome; Inhibition effect; MAPK signaling pathway; Molecular structure of glutamate; Protein.

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