2. Academic Validation
  3. Live-cell imaging and analysis of 3D spheroids in hypoxia- and radiotherapy-related research

Live-cell imaging and analysis of 3D spheroids in hypoxia- and radiotherapy-related research

  • Clin Transl Radiat Oncol. 2025 Jan 15:51:100920. doi: 10.1016/j.ctro.2025.100920.
Claire Beckers 1,  Lazaros Vasilikos 1,  Lorena Moor 1,  Martin Pruschy 1
Affiliations

Affiliation

  • 1 Laboratory for Applied Radiobiology, Department of Radiation Oncology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
PMID: 39898333 DOI: 10.1016/j.ctro.2025.100920
Abstract

3D spheroids are a valuable tool for investigating the interplay between hypoxia and radioresistance. However, standardized methods for visualizing normoxic and hypoxic regions within spheroids and assessing treatment responses are lacking. We developed a straightforward workflow for the analysis of spheroids based on image processing using a novel custom-written script, and flow cytometry.

Keywords

3D cell culture; Flow cytometry; Fluorescence microscopy; Hypoxia; Radiotherapy; Spheroids.

Figures
Products