2. Academic Validation
  3. Trps1 regulates mouse zygotic genome activation and preimplantation embryo development via the PDE4D/AKT/CREB signaling pathway

Trps1 regulates mouse zygotic genome activation and preimplantation embryo development via the PDE4D/AKT/CREB signaling pathway

  • Cell Biol Toxicol. 2025 Feb 20;41(1):48. doi: 10.1007/s10565-025-09999-1.
Xia Jiang # 1,  Weiwei Xu # 1,  Jiandong Sun # 1 2 3 4,  Jianmin Lin 1,  Zihang Lin 1,  Xiuli Lian 1 2,  Shumin Liao 1,  Shanshan Luo 1,  Yue Liu 5 6,  Shie Wang 7 8
Affiliations

Affiliations

  • 1 Key Laboratory of Stem Cell Engineering and Regenerative Medicine of Fujian Province University, Fujian Medical University, Fuzhou, 350122, People's Republic of China.
  • 2 Department of Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, People's Republic of China.
  • 3 Department of Andrology, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350122, People's Republic of China.
  • 4 Department of Reproductive Medicine Centre, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350122, People's Republic of China.
  • 5 Key Laboratory of Stem Cell Engineering and Regenerative Medicine of Fujian Province University, Fujian Medical University, Fuzhou, 350122, People's Republic of China. 331042317@qq.com.
  • 6 Department of Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, People's Republic of China. 331042317@qq.com.
  • 7 Key Laboratory of Stem Cell Engineering and Regenerative Medicine of Fujian Province University, Fujian Medical University, Fuzhou, 350122, People's Republic of China. shiewang@fjmu.edu.cn.
  • 8 Department of Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, People's Republic of China. shiewang@fjmu.edu.cn.
  • # Contributed equally.
PMID: 39979480 DOI: 10.1007/s10565-025-09999-1
Abstract

Despite zygotic genome activation (ZGA) is crucial for early embryonic development, its regulatory mechanism is still unclear in mammals. In the present study, we demonstrate that TRPS1, a maternal factor, plays an essential role in mouse early embryogenesis by regulating the transition from 2-cell to 4-cell embryos during preimplantation development. The absence of Trps1 could leads to impaired ZGA through Akt/CREB signaling pathway. Furthermore, our findings suggest that TRPS1 may modulate the transcription of Pde4d to influence Akt and CREB phosphorylation. Interestingly, compared to Trps1 knockdown alone, co-injection of Trps1 siRNA and Pde4d mRNA significantly enhances the development rate of 4-cell embryos. Collectively, these results indicate a negative involvement of Trps1 in mouse preimplantation embryo development by targeting the PDE4D/Akt/CREB pathway to regulate ZGA.

Keywords

AKT/CREB pathway; Phosphodiesterase 4d; Tricho-rhino-phalangeal syndrome 1; Zygotic genome activation.

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