2. Academic Validation
  3. Human leukocyte interferon produced by E. coli is biologically active

Human leukocyte interferon produced by E. coli is biologically active

  • Nature. 1980 Oct 2;287(5781):411-6. doi: 10.1038/287411a0.
D V Goeddel,  E Yelverton,  A Ullrich,  H L Heyneker,  G Miozzari,  W Holmes,  P H Seeburg,  T Dull,  L May,  N Stebbing,  R Crea,  S Maeda,  R McCandliss,  A Sloma,  J M Tabor,  M Gross,  P C Familletti,  S Pestka
PMID: 6159538 DOI: 10.1038/287411a0
Abstract

A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli. The DNA sequence codes for a 23-amino acid signal peptide followed by an interferon polypeptide of 165 Amino acids. An expression plasmid was constructed which permits the synthesis in E. coli of 2.5 x 10(8) units of interferon per litre of culture. This LeIF protected squirrel monkeys from lethal encephalomyocarditis virus Infection.

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