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  3. A rapid and simple procedure for the preparation of human lactate dehydrogenase-2 from erythrocytes using an ion-exchange mini-column

A rapid and simple procedure for the preparation of human lactate dehydrogenase-2 from erythrocytes using an ion-exchange mini-column

  • Clin Chim Acta. 1980 Jan 1;100(1):59-63. doi: 10.1016/0009-8981(80)90186-2.
S Pettit,  D A Nealon,  A R Henderson
PMID: 7351079 DOI: 10.1016/0009-8981(80)90186-2
Abstract

We describe a simple ion-exchange chromatography technique for the rapid preparation of lactate dehydrogenase-2 from lysed human erythrocytes obtained either from plasma or serum clots. The procedure appears capable of scale-up to larger columns thus making it ideal as an initial step in a more extensive purification scheme.

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