Expression of human thromboxane synthase using a baculovirus system

FEBS Lett. 1993 Feb 22;318(1):91-4. doi: 10.1016/0014-5793(93)81335-w.

C Yokoyama ¹, A Miyata, K Suzuki, Y Nishikawa, T Yoshimoto, S Yamamoto, R Nüsing, V Ullrich, T Tanabe

Affiliations

Affiliation

1 Department of Pharmacology, National Cardiovascular Center Research Institute, Osaka, Japan.

PMID: 8436233 DOI: 10.1016/0014-5793(93)81335-w

Abstract

Human thromboxane (TX) synthase (EC 5.3.99.5) was produced by the baculovirus expression system using cDNA encoding human TX synthase [(1991) Biochem. Biophys. Res. Commun. 78, 1479-1484]. A recombinant baculovirus TXS7 was expressed in Spodoptera frugiperda Sf9 insect cells. The expressed protein was recognized by monoclonal antibody, Kon 7 raised against human TX synthase [(1990) Blood 76, 80-85]. The recombinant TX synthase catalyzed the conversion of prostaglandin (PG) H2 to TXA2 and 12-hydroxy-heptadecatrienoic acid (HHT). Both conversions of PGH2 to TXA2 and HHT by the expressed TX synthase were completely inhibited by a specific TX synthase inhibitor, OKY-046 (5 microM).

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