2. Academic Validation
  3. Phosphorylation of Ser-42 and Ser-59 in the N-terminal region of the tyrosine kinase p56lck

Phosphorylation of Ser-42 and Ser-59 in the N-terminal region of the tyrosine kinase p56lck

  • Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5176-80. doi: 10.1073/pnas.90.11.5176.
D G Winkler 1,  I Park,  T Kim,  N S Payne,  C T Walsh,  J L Strominger,  J Shin
Affiliations

Affiliation

  • 1 Division of Tumor Virology, Dana-Farber Cancer Institute, Boston, MA 02115.
PMID: 8506364 DOI: 10.1073/pnas.90.11.5176
Abstract

Ser-42 and Ser-59 in the N-terminal region have been identified as the major phorbol ester-induced phosphorylation sites of p56lck. Phosphorylation of Ser-59 results in a gel shift from 56 kDa to 61 kDa. Simultaneous phosphorylation of Ser-42 and Ser-59 results in a further gel shift to 63 kDa. In vitro kinase assays show that Ser-59 can be uniquely phosphorylated by mitogen-activated protein kinase and that Ser-42 can be phosphorylated by either protein kinase A or protein kinase C.

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