2. Academic Validation
  3. The alpha(1,3)fucosyltransferase Fuc-TVII controls leukocyte trafficking through an essential role in L-, E-, and P-selectin ligand biosynthesis

The alpha(1,3)fucosyltransferase Fuc-TVII controls leukocyte trafficking through an essential role in L-, E-, and P-selectin ligand biosynthesis

  • Cell. 1996 Aug 23;86(4):643-53. doi: 10.1016/s0092-8674(00)80137-3.
P Malý 1,  A Thall,  B Petryniak,  C E Rogers,  P L Smith,  R M Marks,  R J Kelly,  K M Gersten,  G Cheng,  T L Saunders,  S A Camper,  R T Camphausen,  F X Sullivan,  Y Isogai,  O Hindsgaul,  U H von Andrian,  J B Lowe
Affiliations

Affiliation

  • 1 Howard Hughes Medical Institute, University of Michigan Medical School, Ann Arbor 48109-0650, USA.
PMID: 8752218 DOI: 10.1016/s0092-8674(00)80137-3
Abstract

alpha(1,3)Fucosylated oligosaccharides represent components of leukocyte counterreceptors for E- and P-selectins and of L-selectin ligands expressed by lymph node high endothelial venules (HEV). The identity of the alpha(1,3)fucosyltransferase(s) required for their expression has been uncertain, as has a requirement for alpha(1,3)fucosylation in HEV L-selectin ligand activity. We demonstrate here that mice deficient in alpha(1,3) fucosyltransferase Fuc-TVII exhibit a leukocyte adhesion deficiency characterized by absent leukocyte E- and P-selectin ligand activity and deficient HEV L-selectin ligand activity. Selectin ligand deficiency is distinguished by blood leukocytosis, impaired leukocyte extravasation in inflammation, and faulty lymphocyte homing. These observations demonstrate an essential role for Fuc-TVII in E-, P-, and L-selectin ligand biosynthesis and imply that this locus can control leukocyte trafficking in health and disease.

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