2. Academic Validation
  3. The 400 kDa subunit of the PCAF histone acetylase complex belongs to the ATM superfamily

The 400 kDa subunit of the PCAF histone acetylase complex belongs to the ATM superfamily

  • Mol Cell. 1998 Dec;2(6):869-75. doi: 10.1016/s1097-2765(00)80301-9.
A Vassilev 1,  J Yamauchi,  T Kotani,  C Prives,  M L Avantaggiati,  J Qin,  Y Nakatani
Affiliations

Affiliation

  • 1 Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
PMID: 9885574 DOI: 10.1016/s1097-2765(00)80301-9
Abstract

PCAF histone acetylase is found in a complex with more than 20 associated polypeptides. Here we report cloning and characterization of the 400 kDa PCAF-associated factor referred to as PAF400. PAF400 is almost identical to TRRAP, which binds to c-Myc and E2F, and has significant sequence similarities to the ATM superfamily including FRAP, ATM, ATR, and the catalytic subunit of DNA-PK. Remarkably, PAF400 and FRAP share sequence similarity in broad regions that cover 80% of the entire PAF400 sequence. However, unlike the other members of the ATM superfamily, PAF400 is not a protein kinase as judged from the lack of kinase motif and autophosphorylation activity. We discuss the possibility that PAF400 may play a role in signaling of DNA damage to p53 by stimulation of p53 acetylation.

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