Methyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate

Cat. No.: HY-W030319 Purity: 97.39%: FAQs
Data Sheet SDS COA Handling Instructions Technical Support

Methyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate, a natural coumarins derivative, is exploited for the synthesis of the switchable fluorescent substrates to be used in bacterial enzyme detection.

For research use only. We do not sell to patients.

Methyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate Chemical Structure

CAS No. : 15991-13-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Purity & Documentation
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Description

Methyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate, a natural coumarins derivative, is exploited for the synthesis of the switchable fluorescent substrates to be used in bacterial enzyme detection^[1]^[2]^[3].

In Vitro

Methyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate (compound 2) exhibits the maximum absorption (λabs~322 nm) and emission (λem~387 nm) wavelengths^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

234.20

Formula

C₁₂H₁₀O₅

CAS No.

15991-13-6

Appearance

Solid

Color

White to light yellow

SMILES

O=C1OC(C=C(O)C=C2)=C2C(CC(OC)=O)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 116.67 mg/mL (498.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.2699 mL	21.3493 mL	42.6985 mL
5 mM	0.8540 mL	4.2699 mL	8.5397 mL
10 mM	0.4270 mL	2.1349 mL	4.2699 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 97.39%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (251 KB) HNMR (142 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Nascimento JSD, et al. Mapping the Biotransformation of Coumarins through Filamentous Fungi. Molecules. 2019 Sep 29;24(19). [Content Brief]

[2]. Lizzul-Jurse A, et al. Readily functionalizable phosphonium-tagged fluorescent coumarins for enhanced detection of conjugates by mass spectrometry. Org Biomol Chem. 2016 Aug 10;14(32):7777-91. [Content Brief]

[3]. Giovannini G, et al. Coumarin-based, switchable fluorescent substrates for enzymatic bacterial detection. Talanta. 2018 Oct 1;188:448-453. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.2699 mL	21.3493 mL	42.6985 mL	106.7464 mL
	5 mM	0.8540 mL	4.2699 mL	8.5397 mL	21.3493 mL
	10 mM	0.4270 mL	2.1349 mL	4.2699 mL	10.6746 mL
	15 mM	0.2847 mL	1.4233 mL	2.8466 mL	7.1164 mL
	20 mM	0.2135 mL	1.0675 mL	2.1349 mL	5.3373 mL
	25 mM	0.1708 mL	0.8540 mL	1.7079 mL	4.2699 mL
	30 mM	0.1423 mL	0.7116 mL	1.4233 mL	3.5582 mL
	40 mM	0.1067 mL	0.5337 mL	1.0675 mL	2.6687 mL
	50 mM	0.0854 mL	0.4270 mL	0.8540 mL	2.1349 mL
	60 mM	0.0712 mL	0.3558 mL	0.7116 mL	1.7791 mL
	80 mM	0.0534 mL	0.2669 mL	0.5337 mL	1.3343 mL
	100 mM	0.0427 mL	0.2135 mL	0.4270 mL	1.0675 mL