ML-9 Free Base 

ML-9 (Free Base) is a selective and potent inhibitor of Akt kinase, inhibits myosin light-chain kinase (MLCK) and stromal interaction molecule 1 (STIM1) activity^[3]. ML-9 (Free Base) inhibits inhibits MLCK, PKA and PKC activity with K_i values of 4, 32 and 54 μM, respectively^[1]. ML-9 (Free Base) induces autophagy by stimulating autophagosome formation and inhibiting their degradation^[3].

ML9 (Free Base) (0-100 μM; 0-24 hours) has no reduction in cardiomyocyte viability, 50-100 μM significantly induces cell death^[2].
ML9 (Free Base) (50 μM; 1-4 hours) significantly increases cleaved caspase-3 levels, decreased STIM1 protein levels by about 42%^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

324.83

C₁₅H₁₇ClN₂O₂S

110448-31-2

O=S(N1CCNCCC1)(C2=C3C=CC=C(C3=CC=C2)Cl)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ito S, et al. ML-9, a myosin light chain kinase inhibitor, reduces intracellular Ca2+ concentration in guinea pig trachealis.Eur J Pharmacol. 2004 Feb 23;486(3):325-33.  [Content Brief]

  [2]. Shaikh S, et al. The STIM1 inhibitor ML9 disrupts basal autophagy in cardiomyocytes by decreasing lysosome content.Toxicol In Vitro. 2018 Apr;48:121-127.  [Content Brief]

  [3]. Kondratskyi A1, et al.Identification of ML-9 as a lysosomotropic agent targeting autophagy and cell death.Cell Death Dis. 2014 Apr 24;5:e1193.  [Content Brief]