ML-9

Name: ML-9
Brand: MedChemExpress
SKU: HY-100932
Rating: 5.0 (4 reviews)

Cat. No.: HY-100932 Purity: 99.86%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

ML-9 is a selective and potent inhibitor of Akt kinase, inhibits myosin light-chain kinase (MLCK) and stromal interaction molecule 1 (STIM1) activity. ML-9 inhibits inhibits MLCK, PKA and PKC activity with K_i values of 4, 32 and 54?μM, respectively. ML-9 induces autophagy by stimulating autophagosome formation and inhibiting their degradation.

For research use only. We do not sell to patients.

ML-9 Chemical Structure

CAS No. : 105637-50-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 48	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 48	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 44	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 66	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 175	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 260	In-stock	Estimated Time of Arrival: December 31
250 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
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1 g		Get quote

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Based on 4 publication(s) in Google Scholar

Other Forms of ML-9:

ML-9 Free Base Get quote

4 Publications Citing Use of MCE ML-9

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Biological Activity
Purity & Documentation
References
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Description

ML-9 is a selective and potent inhibitor of Akt kinase, inhibits myosin light-chain kinase (MLCK) and stromal interaction molecule 1 (STIM1) activity^[3]. ML-9 inhibits inhibits MLCK, PKA and PKC activity with K_i values of 4, 32 and 54?μM, respectively^[1]. ML-9 induces autophagy by stimulating autophagosome formation and inhibiting their degradation^[3].

In Vitro

ML9 (0-100 μM; 0-24 hours) has no reduction in cardiomyocyte viability, 50-100 μM significantly induces cell death^[2].
ML9 (50 μM; 1-4 hours) significantly increases cleaved caspase-3 levels, decreased STIM1 protein levels by about 42%^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Neonatal rat ventricular myocytes (NRVM) cells
Concentration:	0, 10, 50 and 100 μM
Incubation Time:	0, 1, 4, 8 and 24 hours
Result:	Decreased cell viability at 50-100 μM concentration.

Apoptosis Analysis^[1]

Cell Line:	Neonatal rat ventricular myocytes (NRVM) cells
Concentration:	50 μM
Incubation Time:	1, 4 and 8 hours
Result:	Induced cardiomyocyte death through necrosis and apoptosis.

Molecular Weight

361.29

Formula

C₁₅H₁₈Cl₂N₂O₂S

CAS No.

105637-50-1

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=S(N1CCNCCC1)(C2=C3C=CC=C(Cl)C3=CC=C2)=O.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (230.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 5 mg/mL (13.84 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7679 mL	13.8393 mL	27.6786 mL
5 mM	0.5536 mL	2.7679 mL	5.5357 mL
10 mM	0.2768 mL	1.3839 mL	2.7679 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.76 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.76 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.76 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.86%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (252 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Ito S, et al. ML-9, a myosin light chain kinase inhibitor, reduces intracellular Ca2+ concentration in guinea pig trachealis.Eur J Pharmacol. 2004 Feb 23;486(3):325-33. [Content Brief]

[2]. Shaikh S, et al. The STIM1 inhibitor ML9 disrupts basal autophagy in cardiomyocytes by decreasing lysosome content.Toxicol In Vitro. 2018 Apr;48:121-127. [Content Brief]

[3]. Kondratskyi A1, et al.Identification of ML-9 as a lysosomotropic agent targeting autophagy and cell death.Cell Death Dis. 2014 Apr 24;5:e1193. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.7679 mL	13.8393 mL	27.6786 mL	69.1965 mL
	5 mM	0.5536 mL	2.7679 mL	5.5357 mL	13.8393 mL
	10 mM	0.2768 mL	1.3839 mL	2.7679 mL	6.9196 mL
DMSO	15 mM	0.1845 mL	0.9226 mL	1.8452 mL	4.6131 mL
	20 mM	0.1384 mL	0.6920 mL	1.3839 mL	3.4598 mL
	25 mM	0.1107 mL	0.5536 mL	1.1071 mL	2.7679 mL
	30 mM	0.0923 mL	0.4613 mL	0.9226 mL	2.3065 mL
	40 mM	0.0692 mL	0.3460 mL	0.6920 mL	1.7299 mL
	50 mM	0.0554 mL	0.2768 mL	0.5536 mL	1.3839 mL
	60 mM	0.0461 mL	0.2307 mL	0.4613 mL	1.1533 mL
	80 mM	0.0346 mL	0.1730 mL	0.3460 mL	0.8650 mL
	100 mM	0.0277 mL	0.1384 mL	0.2768 mL	0.6920 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

ML-9 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ML-9105637-50-1ML9ML 9MyosinMLCKSTIM1cardiomyocyteviabilitycaspaseInhibitorinhibitorinhibit

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ML-9

Customer Review

Other Forms of ML-9:

ML-9 Related Antibodies

4 Publications Citing Use of MCE ML-9

Featured Recommendations

•Related Screening Libraries:

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•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

ML-9 Related Antibodies

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Complete Stock Solution Preparation Table

ML-9 Related Classifications

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