MOPS 

MOPS are commonly used as buffers in biology. MOPS buffer maintains the pH of mammalian cell culture media. MOPS and Tris buffer also have inhibitory effects on TfCut2 and LCC hydrolases, inhibiting the rate of hydrolyzing PET films. MOPS may also interfere with calcium binding, translocation, and utilization in vascular smooth muscle cells^[1][2][3][4].

Preparation of Keratinocyte and Microbial Culture Medium Using MOPS^[3]
Materials:
MOPS: 164 mM
RPMI-1640 medium
L-glutamine: 2 mM
Sodium bicarbonate: 2.0 g/L
Sterile phosphate-buffered saline (PBS, pH 7.2)
Normal oral keratinocytes (NOK) or human keratinocyte cell line (HaCat)
Microorganisms: Candida albicans SC5314, Staphylococcus aureus ATCC25923
Sterile water
Steps:
1. Preparation of MOPS Buffer:
Dissolve 164 mM of MOPS in 1 L of sterile water, and adjust the pH to 7.0.
Add 2 mM of L-glutamine and 2.0 g/L of sodium bicarbonate, stirring until fully dissolved.
Filter the solution using a sterile filtration device to ensure sterility.
2. Preparation of Keratinocyte Culture Medium:
Mix the prepared MOPS buffer with RPMI-1640 medium at a 1:1 ratio to obtain MOPS-containing medium.
Seed NOK or HaCat cells into a 24-well plate, adding approximately 4.5 × 10⁴ cells per well with an appropriate amount of RPMI/MOPS medium.
Incubate the cells in a 37°C, 5% CO₂ incubator until confluent (approximately 48 hours).
3. Preparation of Microbial Culture Medium:
Prepare the Candida albicans and Staphylococcus aureus strains, culturing them in suitable media until they reach the logarithmic growth phase.
Resuspend the microorganisms in MOPS medium at a concentration of approximately 10⁷ cells/mL.
Inoculate the microorganisms into a 24-well plate, adding an appropriate amount of MOPS medium, and incubate at 37°C in a shaker at 75 rpm for 90 minutes.
4. Co-culture of Keratinocytes and Microorganisms:
Co-culture keratinocytes and microorganisms in a 24-well plate, adding MOPS medium to maintain the pH around 7.0.
The co-culture duration can be set based on experimental needs, with a recommendation to limit the time to under 12 hours to reduce the cytotoxicity of MOPS.
5. Cell Viability Assay:
Use the MTT assay to measure cell viability, sampling every 2 hours, adding MTT/PBS solution, incubating at 37°C for 4 hours, and recording the results.
Notes:
MOPS is relatively safe for short-term experiments, but it may significantly reduce cell viability after 4 hours, so it is recommended to limit the experiment duration to 4-12 hours.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

209.26

C₇H₁₅NO₄S

1132-61-2

Solid

White to off-white

O=S(CCCN1CCOCC1)(O)=O

Room temperature in continental US; may vary elsewhere.

RT, protect from light

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Steven D Carson, et al. MOPS and coxsackievirus B3 stability. Virology. 2017 Jan 15;501:183-187.  [Content Brief]

  [2]. Juliane Schmidt, et al. Effect of Tris, MOPS, and phosphate buffers on the hydrolysis of polyethylene terephthalate films by polyester hydrolases. FEBS Open Bio. 2016 Jul 20;6(9):919-27.  [Content Brief]

  [3]. Dias Kde C, et al. Influence of different buffers (HEPES/MOPS) on keratinocyte cell viability and microbial growth. J Microbiol Methods. 2016 Jun;125:40-2.  [Content Brief]