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  3. N-Succinimidyl bromoacetate

N-Succinimidyl bromoacetate  (Synonyms: NHS-Bromoacetate)

Cat. No.: HY-W012166 Purity: ≥98.0%
Handling Instructions Technical Support

N-Succinimidyl bromoacetate is a heterobifunctional cross-linking reagent can be used to prepare peptide-protein immunogens.

For research use only. We do not sell to patients.

N-Succinimidyl bromoacetate Chemical Structure

N-Succinimidyl bromoacetate Chemical Structure

CAS No. : 42014-51-7

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Description

N-Succinimidyl bromoacetate is a heterobifunctional cross-linking reagent can be used to prepare peptide-protein immunogens[1].

Molecular Weight

236.02

Formula

C6H6BrNO4

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(CBr)ON1C(CCC1=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (423.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.2369 mL 21.1846 mL 42.3693 mL
5 mM 0.8474 mL 4.2369 mL 8.4739 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥98.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.2369 mL 21.1846 mL 42.3693 mL 105.9232 mL
5 mM 0.8474 mL 4.2369 mL 8.4739 mL 21.1846 mL
10 mM 0.4237 mL 2.1185 mL 4.2369 mL 10.5923 mL
15 mM 0.2825 mL 1.4123 mL 2.8246 mL 7.0615 mL
20 mM 0.2118 mL 1.0592 mL 2.1185 mL 5.2962 mL
25 mM 0.1695 mL 0.8474 mL 1.6948 mL 4.2369 mL
30 mM 0.1412 mL 0.7062 mL 1.4123 mL 3.5308 mL
40 mM 0.1059 mL 0.5296 mL 1.0592 mL 2.6481 mL
50 mM 0.0847 mL 0.4237 mL 0.8474 mL 2.1185 mL
60 mM 0.0706 mL 0.3531 mL 0.7062 mL 1.7654 mL
80 mM 0.0530 mL 0.2648 mL 0.5296 mL 1.3240 mL
100 mM 0.0424 mL 0.2118 mL 0.4237 mL 1.0592 mL
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N-Succinimidyl bromoacetate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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N-Succinimidyl bromoacetate
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